Team:Manchester/Medals
Registered for iGEM, had a great summer, and will be attending the Giant Jamboree.
Created our team wiki page to document our project.
Attributed all of the work done for our project. This is evidenced on our attributions page.
We will present a poster of our project and give a presentation at the Giant Jamboree.
Completed all safety forms required.
Completed the judging form.
Created and documented Part pages on the Registry for the Parts we have made.
Submitted DNA samples of our new Parts to the Registry.
Participated in the interlab study, and documented are results here.
Contributed an improvement in the characterisation of existing BioBricks BBa_J23105, BBa_J23108, and BBa_J23104.
Validated Part: (BBa_K2213000 (LacUV5_EutS), BBa_K2213001 (TetR_EutMN), BBa_K2213002 (araBAD_EutLK), BBa_K2213003 (cgPPK2_His6), BBa_K2213004 (PduD(1-20)_cgPPK2_mCherry_His6), and BBa_K2213005 (PduD(1-20)_mCherry_cgPPK2_His6).
Collaboration: This year we have collaborated with 12 iGEM teams, details of which can be found on our collaborations page 
Some of our contributions include filling in surveys for LMU, TU Munich, Paris Saclay and the UCL iGEM teams and providing technical support and equipment for the City of London high school team.
Human Practices:
Integrated Human Practices
Improve a previous part or project: We have improved 5 parts in our project. All of our parts can be found here:
- BBa_C0062 - luxR repressor/activator
- BBa_C0071 - rhlR repressor/activator from P. aeruginosa PA3477
- BBa_C0079 - lasR activator from P. aeruginosa PAO1
- BBa_C0077 - cinR activator from Rhizobium leguminosarum
- BBa_K1321333 - pSB-AraC-pBAD
In our proof of concept, we transformed two cell populations with different chromoproteins. The cells expressing the chromoproteins were mixed to form new colours from various co-cultures. We also found that different chromoproteins placed varied loads on the the cell populations, resulting in different growth rates, so that, if grown together, one coloured population would likely outcompete the other. Our Genetically Engineered Artificial Ratio (G.E.A.R.) system could work to maintain the colour of the co-culture by slowing the growth of the faster growing population.
