Team:SUSTech Shenzhen/Results/Behavior
Results
Project
Ethological Experiment Results
For the Odr10::CoChR::GEM-GECO::mCherry worms and str1::Chrimson::GEM-GECO::GFP worms we induced them to do some specific behaviors by the blue and red lights on the NGM plate. We could induce the Odr10::CoChR::GEM-GECO::mCherry worm to crawl a circle on the NGM plate by the blue light (Fig.11).
Then, the Odr10::CoChR::GEM-GECO::mCherry worms were trained by the alcohol. We added the alcohol with different concentration on the NGM plate to form a liquid film and we stimulated the C. elegans in the plate at the same time. After 2 hours we washed the plate to recover the worms in M9 buffer and put the mixture on one side of a new plate. Finally, we put the alcohol on the other side to see if the worms have the tropism of the alcohol.
Then we observed that some of the Odr10::CoChR::GEM-GECO::mCherry worms could swim to the alcohol but the wild type worms could not sense the alcohol (Fig.12). We could get that the Odr10::CoChR::GEM-GECO::mCherry worms (experimental worms) after training had the tropism of the alcohol (Fig.12 A).
We did 3 groups of the control experiments, we could prove that the experimental worms without training (Fig.12 B), the wild type worms after training (Fig.12 C) could not sense the alcohol and the experimental worms after training could not sense the water (Fig.12 D). Furthermore, we did the starvation treatment to our worms before the experiments, because of the hunger the worms would huddle together, however our experimental worms swam to the alcohol (Fig.12 A)) which means that they sensed something they prefer at that time. In our experiments, we could know that our training really made the experimental worms fill in love with the alcohol. After several hours they would still huddle together because the disappearance of alcohol order (Fig.12 E, F). In our experiments, we could know that our training really made the experimental worms fill in love with the alcohol.
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