Team:MIT/REST-results

• ABOUT US
  Team Attributions
• BACKGROUND
  Alternative Splicing CRISPR RNA Binding Proteins REST
• PROJECT
  Guides and ASOs mKate-FF4 2 Exon mKate HBG 3 Exon mKate HBG 3 Exon Dual Fluorescence
• LAB WORK
  Parts/Improved Parts Protocols Notebook Future Work
• MODELLING
• HUMAN PRACTICES
  Integrated HP Public Engagement Collaborations InterLab
• AWARDS
  HP Silver HP Gold Education & Public Engagement Model Parts Collection

Experiments with Small Cell Lung Cancer

Once we had decided on a disease model, namely Small Cell Lung Cancer, we needed an in-vivo way of testing our system. To this end we obtained the H82 Cell line from ATCC.

Preliminary Results

The first thing we had to ensure was that we were able to get DNA into the new cell line. We performed a Viafect optimization to test what our ratio of plasmid to transfection reagent should be. We transfected constitutively expressed BFP and reagent at ratios 1:1, 1:2, 1:3 and 1:4.

Viafect Optimization

Ratio of Viafect:BFP vs. the blue fluorescent output (AU) of the H82s

We see the most fluorescent output at a 1:3 ratio of Viafect:BFP. Moving forward, we transfected at that ratio.