Team:MIT/notebook

• ABOUT US
  Team Attributions
• BACKGROUND
  Alternative Splicing CRISPR RNA Binding Proteins REST
• PROJECT
  Guides and ASOs mKate-FF4 2 Exon mKate HBG 3 Exon mKate HBG 3 Exon Dual Fluorescence
• LAB WORK
  Parts/Improved Parts Protocols Notebook Future Work
• MODELLING
• HUMAN PRACTICES
  Integrated HP Public Engagement Collaborations InterLab
• AWARDS
  HP Silver HP Gold Education & Public Engagement Model Parts Collection

Notebook

The plasmid construction process was complicated; however, the following lab summary compiles the many steps taken over the summer to get where we are now. Many of the protocols mentioned can be seen here.

Once we had built our plasmids, we needed to transiently transfect them into mammalian cells. Below is a spreadsheet of all the transfections we ran: