Team:MIT/protocols
ABOUT US
Team
Attributions
BACKGROUND
Alternative Splicing
CRISPR
RNA Binding Proteins
REST
PROJECT
Guides and ASOs
mKate-FF4
2 Exon mKate HBG
3 Exon mKate HBG
3 Exon Dual Fluorescence
LAB WORK
Parts/Improved Parts
Protocols
Notebook
Future Work
MODELLING
HUMAN PRACTICES
Integrated HP
Public Engagement
Collaborations
InterLab
AWARDS
HP Silver
HP Gold
Education & Public Engagement
Model
Parts Collection
Protocols
Bacterial Lab
Transformation of E. coli
Sequencing
Sample Preparation for Flow Cytometry
Resuspending Primers
Resuspending gBlocks
Primer Design
Pouring LB Agar Plates
PCR
Overnight liquid cultures
Nanodrop
Miniprep
LR Reaction
Golden Gate Assembly
Glycerol Stock
Gel Extraction
Gel Electrophoresis
Diagnostic Restriction Digestion
Annealing Oligonucleotides
Pouring an Agarose Gel
Midiprep
Tissue Culture
Splitting HEK-293 Cells
Splitting NCI-H82 Cells
DNA Transfection into HEK Cells
Seeding Plates
Planning Mamallian Transfections
Interpreting Cytometry Data in CytoFlow
Protocols for REST
RNA Isolation
Reverse Transcription
qPCR
