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             <h3 class='text-center' style='margin-bottom:30px'>Abstract</h3>
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         <div class="container section-wrapper" style="padding-top:0px !important;width:90%">
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                 <h3>Knock-in of Circular RNA gene in Hepatocellular Carcinoma cells via CRISPR/Cas9</h4>
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Hepatocellular Carcinoma (HCC) is the leading cause of cancer deaths worldwide & ranked first among cancers
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in males and next to breast cancer among females in Egypt - based upon results of National Cancer Registry Program of Egypt-.
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"Grabbing the problem from the roots" is the best way to decently describe the use of CRISPR, a special gene editing technique
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that we will be using to modulate a certain circRNA and adjust its gene expression, which is down-regulated in hepatocellular carcinoma.
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This in consequence modifies miRNA expression thus amending the mRNA gene expression; which is the visible problem in our trials.
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This will lead us to adopt a novel strategy for miRNA suppression by using circRNAs. This is accomplished by utilizing a synthetic circuit to give rise to a springboard in our battle against cancer.
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<h2 class='text-center' style='margin-bottom:30px'>AFCM-Egypt</h3>
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                    <p><img style="float: right;" src="https://static.igem.org/mediawiki/2017/d/d4/CRISPR_PNG.png" alt="" width="679" height="988" /></p>
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<h3 class='text-center'> We strive to excel</h4>
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<h3 dir="ltr" style="text-align: left;"><span style="font-size: 20pt; color: #ffffff;">Competing endogenous RNA network : Potential entrants to gene editing in Hepatocellular Carcinoma</span><br /><br /><br />               
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<a class='btn btn-sub text-center' href='http://www.afcm.ac.eg' target='_blank'>click here</a>
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  <h3 class="text-center" style="text-align: center;"><br /><br /><span style="font-size: 14pt; color: #ffffff;">Abstract</span></h3>
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                    <p style="text-align: left;"><span style="font-size: 14pt; color: #ffffff;">&nbsp;</span></p>
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                  <span style="font-size: 14pt; color: #ffffff;">Hepatocellular Carcinoma (HCC) is the leading cause of cancer deaths worldwide &amp; ranked first among cancers in males and next to breast cancer among females in</span><span style="font-size: 14pt; color: #ffffff;"> Egypt based upon results of National Cancer Registry Program of Egypt. "Grabbing the problem from the roots" is the best way to decently describe the use of CRISPR, a special gene editing technique that we will be using to modulate a certain&nbsp; circRNA and adjust its gene expression, which is down-regulated in hepatocellular carcinoma. This in consequence modifies&nbsp; &nbsp;miRNA expression thus amending the mRNA gene expression; which is the visible problem in our trials.&nbsp; &nbsp;This will lead us to adopt a novel strategy for miRNA suppression by using circRNAs. This is&nbsp; &nbsp; accomplished by utilizing a&nbsp; &nbsp;synthetic circuit to give rise to a springboard in our battle against cancer.</span><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /></h3>
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                    AFCM-Egypt</h2>
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                    <h3 class='text-center'>
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                        A Culture of Discipline and Innovation</h3>
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                        <a href="http://www.afcm.ac.eg/" class="btn btn-primary btn-outline with-arrow" target="_blank"> click here <i class="fa fa-arrow-right"></i></a></div>
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Latest revision as of 20:11, 31 October 2017

A F C M

A F C M

Competing endogenous RNA network : Potential entrants to gene editing in Hepatocellular Carcinoma




Abstract

 

Hepatocellular Carcinoma (HCC) is the leading cause of cancer deaths worldwide & ranked first among cancers in males and next to breast cancer among females in Egypt based upon results of National Cancer Registry Program of Egypt. "Grabbing the problem from the roots" is the best way to decently describe the use of CRISPR, a special gene editing technique that we will be using to modulate a certain  circRNA and adjust its gene expression, which is down-regulated in hepatocellular carcinoma. This in consequence modifies   miRNA expression thus amending the mRNA gene expression; which is the visible problem in our trials.   This will lead us to adopt a novel strategy for miRNA suppression by using circRNAs. This is    accomplished by utilizing a   synthetic circuit to give rise to a springboard in our battle against cancer.














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