Team:AFCM-Egypt/Improve

A F C M

A F C M


Improved parts

 

  1. BBa_K2217013: We tried to improve CAG promoter at BBa_K1179069 by making it RFC 10  CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.


  2. BBa_K2217024: We improved this part BBa_K1119006 by Fusing CMV enhancer to CMV promoter in one composite part to enhance its transcription activity BBa_K2217024. Part characterization and usage can be found at Part Experience.

  3.  BBa_K2217027: tried to improve laci function at BBa_K1179062 regarding inhibition of non-coding RNAs by fusing laci the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site. Part characterization and usage can be found at BBa_K2217027_Experience.

     Laci registered at (BBa_K2217012), was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding.