we designed the composite parts constituting these synthesised fragments
and finally we assembled these composite parts into our two main circuits
designed in order to compare their function at regulating non-coding RNAs.
Basic Parts
Part Name | Type | Description | Length | Link |
---|---|---|---|---|
BBa_K2217000 | Basic | CMV Enhancer | 304 | HERE |
BBa_K2217001 | Basic | Circular RNA hsa-circ-000064 | 139 | HERE |
BBa_K2217002 | Basic | U6 Promoter for Cas9 gRNA | 204 | HERE |
BBa_K2217003 | Basic | gRNA for Cas9 Knock in | 20 | HERE |
BBa_K2217004 | Basic | gRNA scaffold for Cas9 | 76 | HERE |
BBa_K2217005 | Basic | SV40 Terminator | 122 | HERE |
BBa_K2217006 | Basic | CMV Promoter | 204 | HERE |
BBa_K2217007 | Basic | T7 Promoter | 19 | HERE |
BBa_K2217008 | Basic | CYC1 Terminator | 250 | HERE |
BBa_K2217009 | Basic | HDR Template of BBa_K2217001 | 139 | HERE |
BBa_K2217010 | Basic | Chicken B-actin Promoter | 278 | HERE |
BBa_K2217012 | Basic | laci | 1080 | HERE |
BBa_K2217014 | Basic | YFP | 720 | HERE |
BBa_K2217015 | Basic | bGH poly(A) signal | 225 | HERE |
BBa_K2217016 | Basic | lac_operator | 17 | HERE |
BBa_K2217017 | Basic | lac_promoter | 31 | HERE |
BBa_K2217021 | Basic | miRNA-1825 binding site on hsa-circ-00064 | 20 | HERE |