Difference between revisions of "Team:Lethbridge/Composite Part"

 
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<body>
 
<body>
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<div><img src="https://static.igem.org/mediawiki/2017/b/b1/Banner_PAcomposite.png"  class="bannerImg"></div>
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<div id="allContent">
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<br><br>
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<div style="clear: both"></div>
  
 +
<br>
  
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<!--BEGINNING OF ADDED CONTENT-->
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<p class= "text12">All composite parts pages were created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well 2 additional constructs for purification of tRNA<sup>Phe</sup> and TX-TL validation.</p>
−
<partinfo>BBa_K2443003 short</partinfo>
+
  
−
Aspartic acid tRNA synthetase responsible for attaching aspartic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
+
<div style="clear: both"></div>
  
 +
<center>
 +
<h1>Composite Parts Table</h1>
 +
<div class="partTable">
 +
<table id="partTable">
 +
    <th id="col1" style="background-color: #377b7e; color: #ffffff;" id="text12">BioBrick</th>
 +
    <th id="col2" style="background-color: #377b7e; color: #ffffff;" id="text12">Protein</th>
 +
    <th id="col3" style="background-color: #377b7e; color: #ffffff;" id="text12">Full Construct</th>
  
−
<!-- Add more about the biology of this part here
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<!--data rows-->
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===Usage and Biology===
+
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443000" target="_blank">BBa_K2443000</a></td>
 +
  <td class=xl94 id="text10">AlaRS</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-AlaRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443001" target="_blank">BBa_K2443001</a></td>
 +
  <td class=xl95 id="text10">ArgRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-ArgRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443002" target="_blank">BBa_K2443002</a></td>
 +
  <td class=xl94 id="text10">AsnRS</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-AsnRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443003" target="_blank">BBa_K2443003</a></td>
 +
  <td class=xl95 id="text10">AspRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-AspRS-dT</td>
 +
</tr>
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443004" target="_blank">BBa_K2443004</a></td>
 +
  <td class=xl94 id="text10">CysRS</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-CysRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443005" target="_blank">BBa_K2443005</a></td>
 +
  <td class=xl95 id="text10">GlnRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-GlnRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99  id="text10"><a href="http://parts.igem.org/Part:BBa_K2443006" target="_blank">BBa_K2443006</a></td>
 +
  <td class=xl94  id="text10">GluRS</td>
 +
  <td class=xl105  id="text10">pT7-mRBS-GluRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443007" target="_blank">BBa_K2443007</a></td>
 +
  <td class=xl95 id="text10">GlyRS alpha subunit</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-GlyRSAlpha-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443008" target="_blank">BBa_K2443008</a></td>
 +
  <td class=xl94 id="text10">GlyRS beta subunit</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-GlyRSBeta-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443009" target="_blank">BBa_K2443009</a></td>
 +
  <td class=xl95 id="text10">HisRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-HisRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443010" target="_blank">BBa_K2443010</a></td>
 +
  <td class=xl94 id="text10">IleRS</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-IleRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443011" target="_blank">BBa_K2443011</a></td>
 +
  <td class=xl95 id="text10">LeuRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-LeuRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443012" target="_blank">BBa_K2443012</a></td>
 +
  <td class=xl94 id="text10">LysRS</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-LysRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443013" target="_blank">BBa_K2443013</a></td>
 +
  <td class=xl95 id="text10">MetRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-MetRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443014" target="_blank">BBa_K2443014</a></td>
 +
  <td class=xl94 id="text10">PheRS alpha subunit</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-PheRSAlpha-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443015" target="_blank">BBa_K2443015</a></td>
 +
  <td class=xl95 id="text10">PheRS beta subunit</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-PheRSBeta-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443016" target="_blank">BBa_K2443016</a></td>
 +
  <td class=xl94 id="text10">ProRS</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-ProRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443017" target="_blank">BBa_K2443017</a></td>
 +
  <td class=xl95 id="text10">SerRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-SerRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443018" target="_blank">BBa_K2443018</a></td>
 +
  <td class=xl94 id="text10">ThrRS</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-ThrRS-dT</td>
 +
</tr>
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443019" target="_blank">BBa_K2443019</a></td>
 +
  <td class=xl95 id="text10">TrpRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-TrpRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443020" target="_blank">BBa_K2443020</a></td>
 +
  <td class=xl94 id="text10">TyrRS</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-TyrRS-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443021" target="_blank">BBa_K2443021</a></td>
 +
  <td class=xl95 id="text10">ValRS</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-ValRS-dT</td>
 +
</tr>
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443022" target="_blank">BBa_K2443022</a></td>
 +
  <td class=xl94 id="text10">MTF</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-MTF-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443023" target="_blank">BBa_K2443023</a></td>
 +
  <td class=xl95 id="text10">IF1</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-IF1-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443024" target="_blank">BBa_K2443024</a></td>
 +
  <td class=xl94 id="text10">IF2</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-IF2-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443025" target="_blank">BBa_K2443025</a></td>
 +
  <td class=xl95 id="text10">IF3</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-IF3-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443026" target="_blank">BBa_K2443026</a></td>
 +
  <td class=xl94 id="text10">EF-G</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-EFG-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443027" target="_blank">BBa_K2443027</a></td>
 +
  <td class=xl95 id="text10">EF-Tu</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-EFTu-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443028" target="_blank">BBa_K2443028</a></td>
 +
  <td class=xl94 id="text10">EF-Ts</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-EFTs-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443029" target="_blank">BBa_K2443029</a></td>
 +
  <td class=xl95 id="text10">RF1</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-RF1-dT</td>
 +
</tr>
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443030" target="_blank">BBa_K2443030</a></td>
 +
  <td class=xl94 id="text10">RF2</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-RF2-dT</td>
 +
</tr>
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443031" target="_blank">BBa_K2443031</a></td>
 +
  <td class=xl95 id="text10">RF3</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-RF3-dT</td>
 +
</tr>
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443032" target="_blank">BBa_K2443032</a></td>
 +
  <td class=xl94 id="text10">RRF</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-RRF-dT</td>
 +
</tr>
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443033" target="_blank">BBa_K2443033</a></td>
 +
  <td class=xl95 id="text10">MK</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-MK-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443034" target="_blank">BBa_K2443034</a></td>
 +
  <td class=xl94 id="text10">CK</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-CK-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443035" target="_blank">BBa_K2443035</a></td>
 +
  <td class=xl95 id="text10">NDK</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-NDK-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443036" target="_blank">BBa_K2443036</a></td>
 +
  <td class=xl94 id="text10">PPiase</td>
 +
  <td class=xl105 id="text10">pT7-mRBS-PPiase-dT</td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/Part:BBa_K2443037" target="_blank">BBa_K2443037</a></td>
 +
  <td class=xl95 id="text10">T7 RNA Polymerase</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-T7 RNA Polymerase-dT</td>
 +
</tr>
  
−
<!-- -->
+
<tr>
−
<span class='h3bb'>Sequence and Features</span>
+
  <td class=xl99 id="text10"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2443038" target="_blank">BBa_K2443038</a></td>
−
<partinfo>BBa_K2443003 SequenceAndFeatures</partinfo>
+
  <td class=xl94 id="text10">tRNAPhe</td>
 +
  <td class=xl105 id="text10">pT7-tRNAPhe-2xMS2 Hairpin-dT</td>
 +
</tr>  
  
 +
<tr>
 +
  <td class=xl100 id="text10"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2443039" target="_blank">BBa_K2443039</a></td>
 +
  <td class=xl95 id="text10">EYFP-Spinach</td>
 +
  <td class=xl106 id="text10">pT7-mRBS-EYFP-Spinach-dT</td>
 +
</tr>
 +
   
 +
</table>
 +
</div>
 +
</center>
 +
<br>
 +
<br>
  
−
<!-- Uncomment this to enable Functional Parameter display
+
</div><!-- closing #allContent -->
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===Functional Parameters===
+
<img src="https://static.igem.org/mediawiki/2017/7/7d/Banner_footer_blank.png" class="bannerImg">
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<partinfo>BBa_K2443003 parameters</partinfo>
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</body>
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<!-- -->
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</html>

Latest revision as of 03:14, 2 November 2017

top



All composite parts pages were created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well 2 additional constructs for purification of tRNAPhe and TX-TL validation.

Composite Parts Table

BioBrick Protein Full Construct
BBa_K2443000 AlaRS pT7-mRBS-AlaRS-dT
BBa_K2443001 ArgRS pT7-mRBS-ArgRS-dT
BBa_K2443002 AsnRS pT7-mRBS-AsnRS-dT
BBa_K2443003 AspRS pT7-mRBS-AspRS-dT
BBa_K2443004 CysRS pT7-mRBS-CysRS-dT
BBa_K2443005 GlnRS pT7-mRBS-GlnRS-dT
BBa_K2443006 GluRS pT7-mRBS-GluRS-dT
BBa_K2443007 GlyRS alpha subunit pT7-mRBS-GlyRSAlpha-dT
BBa_K2443008 GlyRS beta subunit pT7-mRBS-GlyRSBeta-dT
BBa_K2443009 HisRS pT7-mRBS-HisRS-dT
BBa_K2443010 IleRS pT7-mRBS-IleRS-dT
BBa_K2443011 LeuRS pT7-mRBS-LeuRS-dT
BBa_K2443012 LysRS pT7-mRBS-LysRS-dT
BBa_K2443013 MetRS pT7-mRBS-MetRS-dT
BBa_K2443014 PheRS alpha subunit pT7-mRBS-PheRSAlpha-dT
BBa_K2443015 PheRS beta subunit pT7-mRBS-PheRSBeta-dT
BBa_K2443016 ProRS pT7-mRBS-ProRS-dT
BBa_K2443017 SerRS pT7-mRBS-SerRS-dT
BBa_K2443018 ThrRS pT7-mRBS-ThrRS-dT
BBa_K2443019 TrpRS pT7-mRBS-TrpRS-dT
BBa_K2443020 TyrRS pT7-mRBS-TyrRS-dT
BBa_K2443021 ValRS pT7-mRBS-ValRS-dT
BBa_K2443022 MTF pT7-mRBS-MTF-dT
BBa_K2443023 IF1 pT7-mRBS-IF1-dT
BBa_K2443024 IF2 pT7-mRBS-IF2-dT
BBa_K2443025 IF3 pT7-mRBS-IF3-dT
BBa_K2443026 EF-G pT7-mRBS-EFG-dT
BBa_K2443027 EF-Tu pT7-mRBS-EFTu-dT
BBa_K2443028 EF-Ts pT7-mRBS-EFTs-dT
BBa_K2443029 RF1 pT7-mRBS-RF1-dT
BBa_K2443030 RF2 pT7-mRBS-RF2-dT
BBa_K2443031 RF3 pT7-mRBS-RF3-dT
BBa_K2443032 RRF pT7-mRBS-RRF-dT
BBa_K2443033 MK pT7-mRBS-MK-dT
BBa_K2443034 CK pT7-mRBS-CK-dT
BBa_K2443035 NDK pT7-mRBS-NDK-dT
BBa_K2443036 PPiase pT7-mRBS-PPiase-dT
BBa_K2443037 T7 RNA Polymerase pT7-mRBS-T7 RNA Polymerase-dT
BBa_K2443038 tRNAPhe pT7-tRNAPhe-2xMS2 Hairpin-dT
BBa_K2443039 EYFP-Spinach pT7-mRBS-EYFP-Spinach-dT