Team:Lethbridge/Improve
Contents
Improved Parts
BBa_K2443037 - T7 RNA Polymerase
Original part: BBa_I2032
Submitted by: MIT 2006
designed by: Bartholomew Canton
Rational behind improvements:
BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
BBa_K2443028 - EF-Ts
Original part:BBa_K1906000
Submitted by: XJTLU-China 2016
designed by: Wenbo Xu
Rational behind improvements:
BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
BBa_K2443027 - EF-Tu
Original part: BBa_K1906004
Submitted by: XJTLU-China 2016
designed by:Yuwei Han
Rational behind improvements:
BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
BBa_K2443013 - Met-RS
Original part:BBa_K567015
Submitted by: SJTU-BioX-Shanghai 2011
designed by:You Wang
Rational behind improvements:
BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
