Difference between revisions of "Team:Lethbridge/Parts"
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| − | <p class="text12">The N<i>ex</i>t <i>vivo</i> system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection" id="pageLink">collection of parts</a> that anyone could use to produce their own N<i>ex</i>t <i>vivo</i> system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in <i>E. coli</i>. As such we sought to <a href="https://2017.igem.org/Team:Lethbridge/Improve" id="pageLink">improve these parts</a> for future use. | + | <p class="text12">The N<i>ex</i>t <i>vivo</i> system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection" id="pageLink">collection of parts</a> that anyone could use to produce their own N<i>ex</i>t <i>vivo</i> system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in <i>E. coli</i>. As such we sought to <a href="https://2017.igem.org/Team:Lethbridge/Improve" id="pageLink">improve these parts</a> for future use.</p> |
| − | </p> | + | <p class="text12">In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the N<i>ex</i>t <i>vivo</i> system makes use of a previously submitted MS2BP part (<a href="http://parts.igem.org/Part:BBa_K2109108" id="pageLink">BBa_K2109108</a>).</p> |
| − | <p class="text12">In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the N<i>ex</i>t <i>vivo</i> system makes use of a previously submitted MS2BP part (<a href="http://parts.igem.org/Part:BBa_K2109108" id="pageLink">BBa_K2109108</a>). | + | </div> |
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| − | + | <h2 class="segmentHeader">Part Collection</h2> | |
| − | + | <p class="text12">The <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection" id="pageLink">N<i>ex</i>t <i>vivo</i> part collection</a> consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (<a href="http://partsregistry.org/Part:BBa_I719005" id="pageLink">BBa_I719005</a>), medium RBS (<a href="http://parts.igem.org/Part:BBa_B0034" id="pageLink">BBa_B0034</a>), and double terminator (<a href="http://parts.igem.org/Part:BBa_B0015" id="pageLink">BBa_B0015</a>).</p> | |
| − | <p class="text12">The <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection" id="pageLink">N<i>ex</i>t <i>vivo</i> part collection</a> consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (<a href="http://partsregistry.org/Part:BBa_I719005" id="pageLink">BBa_I719005</a>), medium RBS (<a href="http://parts.igem.org/Part:BBa_B0034" id="pageLink">BBa_B0034</a>), and double terminator (<a href="http://parts.igem.org/Part:BBa_B0015" id="pageLink">BBa_B0015</a>). | + | </div> |
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<img src="https://static.igem.org/mediawiki/2017/f/f4/T--Lethbridge--partcollection.png" width=1000px; height=533px; padding: 30px class="bannerImg" /> | <img src="https://static.igem.org/mediawiki/2017/f/f4/T--Lethbridge--partcollection.png" width=1000px; height=533px; padding: 30px class="bannerImg" /> | ||
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| − | + | <h2 class="segmentHeader">Composite Parts</h2> | |
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<h2 align= "left">General Protein Construct</h2> | <h2 align= "left">General Protein Construct</h2> | ||
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<img src="https://static.igem.org/mediawiki/2017/9/97/T--Lethbridge--generalconstruct.png" width=800px; height=153px; padding: 30px class="bannerImg" /> | <img src="https://static.igem.org/mediawiki/2017/9/97/T--Lethbridge--generalconstruct.png" width=800px; height=153px; padding: 30px class="bannerImg" /> | ||
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<h2 align= "left">General RNA Construct</h2> | <h2 align= "left">General RNA Construct</h2> | ||
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<img src="https://static.igem.org/mediawiki/2017/a/a8/T--Lethbridge--RNAconstruct.png" width=800px; height=153px; padding: 30px class="bannerImg" /> | <img src="https://static.igem.org/mediawiki/2017/a/a8/T--Lethbridge--RNAconstruct.png" width=800px; height=153px; padding: 30px class="bannerImg" /> | ||
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<h2 class="segmentHeader">Improved Parts</h2> | <h2 class="segmentHeader">Improved Parts</h2> | ||
| − | + | <p class="text12">Of our 38 TX-TL components, four were already in the registry of standard biological parts. We decided to improve these parts for future use in our N<i>ex</i>t <i>vivo</i> system and for general use by others. Our improvements are documented <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Improve" id="pageLink">here.</a></p> | |
| − | <p class="text12">Of our 38 TX-TL components, four were already in the registry of standard biological parts. We decided to improve these parts for future use in our N<i>ex</i>t <i>vivo</i> system and for general use by others. Our improvements are documented <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Improve" id="pageLink">here.</a></p> | + | </div> |
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<img src="https://static.igem.org/mediawiki/2017/6/6f/T--Lethbridge--improve4.png" width=1000px; height=231px; padding: 30px class="bannerImg" /> | <img src="https://static.igem.org/mediawiki/2017/6/6f/T--Lethbridge--improve4.png" width=1000px; height=231px; padding: 30px class="bannerImg" /> | ||
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<h2 class="segmentHeader">Basic Parts</h2> | <h2 class="segmentHeader">Basic Parts</h2> | ||
| − | + | <p class="text12">All <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Basic_Part" id="pageLink">basic parts</a> pages created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well as the parts for our tRNA purification and TX-TL validation construct.</p> | |
| − | <p class="text12">All <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Basic_Part" id="pageLink">basic parts</a> pages created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well as the parts for our tRNA purification and TX-TL validation construct.</p> | + | </div> |
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<img src="https://static.igem.org/mediawiki/2017/5/56/T--Lethbridge--cds.png" width=320px; height=69px; padding: 30px class="bannerImg" /> | <img src="https://static.igem.org/mediawiki/2017/5/56/T--Lethbridge--cds.png" width=320px; height=69px; padding: 30px class="bannerImg" /> | ||
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Revision as of 22:36, 29 October 2017
The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts for future use.
In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the Next vivo system makes use of a previously submitted MS2BP part (BBa_K2109108).
Part Collection
The Next vivo part collection consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015).
Composite Parts
General Protein Construct
General RNA Construct
This year we designed 41 composite parts. Of the 41 parts, it contains 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well 3 additional constructs for tRNA purification and TX-TL validation.
Improved Parts
Of our 38 TX-TL components, four were already in the registry of standard biological parts. We decided to improve these parts for future use in our Next vivo system and for general use by others. Our improvements are documented here.
Basic Parts
All basic parts pages created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well as the parts for our tRNA purification and TX-TL validation construct.
