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| − | <h4>References</h4> | + | <p><b>Overview</b></p> |
| − | <hr/> | + | <p style="margin-left: 40px">1. Polyphosphate kinase purification and optimization.</p> |
| − | | + | <p style="margin-left: 40px">2. Eut microcompartment expression.</p> |
| − | <div class="clear" style="padding: 0px 7vw; border: 3px solid transparent; background-color: #fbf9f8!important"> | + | <p style="margin-left: 40px">3. Localization Tag characterization.</p> |
| − | | + | <p style="margin-left: 40px">4. Anderson Promoters characterization.</p> |
| − | <p>Akiyama, M., Crooke, E. and Kornberg, A. (1992). , An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon. <i>The Journal of Biochemistry</i>, 268(1), pp.633-639.</p>
| + | <p style="margin-left: 40px">5. References</p> |
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| − | <p>AS Rose, AR Bradley, Y Valasatava, JM Duarte, A Prlić and PW Rose. Web-based molecular graphics for large complexes. ACM Proceedings of the 21st International Conference on Web3D Technology (Web3D '16): 185-186, 2016. doi:10.1145/2945292.2945324</p>
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| − | <p>AS Rose and PW Hildebrand. NGL Viewer: a web application for molecular visualization. Nucl Acids Res (1 July 2015) 43 (W1): W576-W579 first published online April 29, 2015. doi:10.1093/nar/gkv402</p> | + | |
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| − | <p>Bednarska, N., Schymkowitz, J., Rousseau, F. and Van Eldere, J. (2013). Protein aggregation in bacteria: the thin boundary between functionality and toxicity. Microbiology, 159(Pt_9), pp.1795-1806.</p>
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| − | <p>Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; | + | |
| − | Protein Identification and Analysis Tools on the ExPASy Server;
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| − | (In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005).
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| − | pp. 571-607</p>
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| − | <p>Jakobson, C., Kim, E., Slininger, M., Chien, A. and Tullman-Ercek, D. (2015). Localization of Proteins to the 1,2-Propanediol Utilization Microcompartment by Non-native Signal Sequences Is Mediated by a Common Hydrophobic Motif. <i>Journal of Biological Chemistry</i>, 290(40), pp.24519-24533.</p>
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| − | <p>Kornberg, A. (1995). Inorganic polyphosphate: toward making a forgotten polymer unforgettable. <i>Journal of Bacteriology</i>, 177(3), pp.491-496.</p>
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| − | <p>Kornberg, A., Kornberg, S. and Simms, E. (1956). Metaphosphate synthesis by an enzyme from Escherichia coli. <i>Biochimica et Biophysica Acta</i>, 20, pp.215-227.</p> | + | |
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| − | <p>Liang, M., Frank, S., Lünsdorf, H., Warren, M. and Prentice, M. (2017). Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation in E. coli. <i>Biotechnology Journal</i>, 12(3), p.1600415.</p>
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| − | <p>Lindner, S., Vidaurre, D., Willbold, S., Schoberth, S. and Wendisch, V. (2007). NCgl2620 Encodes a Class II Polyphosphate Kinase in Corynebacterium glutamicum. <i>Applied and Environmental Microbiology</i>, 73(15), pp.5026-5033.</p> | + | |
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| − | <p>Mino, T., van Loosdrecht, M. and Heijnen, J. (1998). Microbiology and biochemistry of the enhanced biological phosphate removal process. <i>Water Research</i>, 32(11), pp.3193-3207.</p>
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| − | <p>Schwede, T. (2003). SWISS-MODEL: an automated protein homology-modeling server. <i>Nucleic Acids Research</i>, 31(13), pp.3381-3385.</p>
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| − | <p>Thain, A., Gaston, K., Jenkins, O. and Clarke, A. (1996). A method for the separation of GST fusion proteins from co-purifying GroEL. <i>Trends in Genetics</i>, 12(6), pp.209-210.</p>
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| − | <a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/ Demonstrate " ><img src="https://static.igem.org/mediawiki/2017/7/7c/T--Manchester--Next_Arrow17.jpg"></a> | + | <a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/ Results1 " ><img src="https://static.igem.org/mediawiki/2017/7/7c/T--Manchester--Next_Arrow17.jpg"></a> |
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| − | <a class="floatright1 project projectlink" href= " https://2017.igem.org/Team:Manchester/ Results4 " ><img src="https://static.igem.org/mediawiki/2017/0/0a/T--Manchester--BACKARROW17MAN.jpg"></a> | + | <a class="floatright1 project projectlink" href= " https://2017.igem.org/Team:Manchester/ Results " ><img src="https://static.igem.org/mediawiki/2017/0/0a/T--Manchester--BACKARROW17MAN.jpg"></a> |
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