Difference between revisions of "Team:SUSTech Shenzhen/Hardware"

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== Projector Light Source ==
 
== Projector Light Source ==
  
We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then CoChR and Chrimson are activated by blue light and red light from LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source in the same time.
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We need 395nm light to activate calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then CoChR and Chrimson are activated by blue light and red light from LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source in the same time.
  
 
{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--IMG_20170320_233120_HDR.jpg|caption= Apparatus to merge projector and microscope. }}
 
{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--IMG_20170320_233120_HDR.jpg|caption= Apparatus to merge projector and microscope. }}
 
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To achieve the aim, we add additional filter to purify the red light and blue light and replace new lens to adjust the focus distance.
 
{{SUSTech_Image | filename=T--SUSTech_Shenzhen--projectorintomicroscope.jpg|width=200px| caption=We project a movie on the hand.}}
 
{{SUSTech_Image | filename=T--SUSTech_Shenzhen--projectorintomicroscope.jpg|width=200px| caption=We project a movie on the hand.}}
We choose projector as red and blue light source, because we can activate single cell or ''<i>C. elegans</i>'' by light of adjustable color and intensity. Because the focus distance of origin lens in projector too short for our microscopy(Nikon Ti-E), a longer focus distance lens is replaced. We install a new lens into projector. Smaller the image of projector, and put image into microscope.
 
 
Protein are sensitive to the wavelength of light, it necessary to purify light of the projector. We buy and replace with new  filter [https://www.chroma.com/products/parts/et480-20x Chroma ET480/20X] and [https://www.chroma.com/products/parts/et630-20x Chroma ET630/20X], both are fixed inside projector. Di-mirror [https://www.chroma.com/products/parts/89402bs 89402bs] and emission filter [https://www.chroma.com/products/parts/89402m 89402m] are been installed in Microscope filter wheel.
 
  
Software is most flexible part. A very easy method is to use a slide([https://static.igem.org/mediawiki/2017/f/f1/T--SUSTech_Shenzhen--slidestimulation.zip Demo Slide]), such as [https://www.libreoffice.org/en LibreOffice](Test on 5.4.2.2.0+ in Arch Linux) or Microsoft Office. The image and time pattern  is stetted in slide, including color, intensity, pulse time etc.
 
  
We try develop a more powerful and hackable open source software suite called ''ColorMapping'' to track and activate multi ''<i>C. elegans</i>s'' or cell independently in one eye-filed. User can modify multi color, intensity, time, locations of light alternately in GUI. ''ColorMapping'' can be found in [https://github.com/JiangXL/ColorMapping GitHub], which still is developing. ''ColorMapping'' contains camera part, projector part, user&calculation part. More detain can be found in GitHub Document and PDF[].
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We try develop a more powerful and hackable open source software suite called ''ColorMapping'' to track and activate multi ''<i>C. elegans</i>s'' or cell independently in one eye-filed. User can modify multi color, intensity, time, locations of light alternately in GUI. ''ColorMapping'' can be found in [https://github.com/JiangXL/ColorMapping GitHub], which still is developing. ''ColorMapping'' contains camera part, projector part, user&calculation part. More detain can be found in GitHub Document.
  
 
== Arduino Modulate Mercury Lamp ==
 
== Arduino Modulate Mercury Lamp ==

Revision as of 09:45, 1 November 2017

Team SUSTC-Shenzhen



Microfluidics

In our experiment, we want to research the neuron activity and behavioral response of Caenorhabditis elegans under a light stimulus of specific wavelength. Thus, we need to choose an appropriate, clear, efficient and convenient platform to study a group of worms as well as an individual one.

Microfluidics has a very tiny channel, which is the best to match with the size of C. elegans. There is no damaging for worms in Microfluidics. We design three parts of microfluidic chips the Selection Chip to choose appropriate sizes of worms, the Gaussian Plate to prove its olfactory receptor neuron pairs are not affected, and the Immobilization Plate to research the response of individual behavior and neuron activity. (Fig.1)

T--SUSTech Shenzhen--Microfluidics--fig1overview.png
Fig. 1 The three chips in our experiment. A) The design of the Selection Chip and the Gaussian Chip. B) The product of the Selection Chip and the Gaussian Chip. C) The design of the Immobilization Chip. D) The product of the Immobilization Chip.

Detailed Microfluidics


Light Modulator

Multiple devices of optics are designed and created for the various experiment requirements, such as, stimulate neuron of C. elegans, train C. elegans and induce C. elegans move in a special direction. All devices attempts to modulate the spatio-temporal pattern in an elegant and effective way. We constructed projector light source and modulate mercury lamp. Just let us start to play with light.

Projector Light Source

We need 395nm light to activate calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then CoChR and Chrimson are activated by blue light and red light from LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source in the same time.


T--SUSTech Shenzhen--IMG 20170320 233120 HDR.jpg
Apparatus to merge projector and microscope.
To achieve the aim, we add additional filter to purify the red light and blue light and replace new lens to adjust the focus distance.
T--SUSTech Shenzhen--projectorintomicroscope.jpg
We project a movie on the hand.


We try develop a more powerful and hackable open source software suite called ColorMapping to track and activate multi C. eleganss or cell independently in one eye-filed. User can modify multi color, intensity, time, locations of light alternately in GUI. ColorMapping can be found in GitHub, which still is developing. ColorMapping contains camera part, projector part, user&calculation part. More detain can be found in GitHub Document.

Arduino Modulate Mercury Lamp

A simple and effective device to output pulse of certain wavelength of light. On time and off time of pulse is custom by Arduino. Wavelength of light is changed by replacing filter before beam expander.

T--SUSTech Shenzhen--DSCF2143.jpg
Overview of Arduino modulate Mercury lamp(A) Arduino transfer the pulse command to servo. (B)The interview of Mercury Lamp.The servo fix at the original bottom. (C) The beam expander connect to stereoscope




Made by from the elegans.Inc in SUSTech_Shenzhen.

Licensed under CC BY 4.0.