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Revision as of 22:38, 1 November 2017
Results
Project
Contents
Microfluidics were divide into three parts: the Selection Chip; the Gaussian Plate; and the Immobilization Chip.
The Selection of the Caenorhabditis elegans
There are two plans of selecting worms. Why we need the C. elegans with the same stage The first one is using microfluidics. We designed the Selection Chip to select the Caenorhabditis elegans (C. elegans) with the appropriate size (Fig.1).
We need a large number of the worms with the same stage to do the Gaussion Plate. Why we need so many with the same stage However, we found that the chip only has 12 fences (Fig.2) The efficiency of the Selection Chip was very low because of such a small number of the fences. In addition, the C. elegans have flexible body, some of the suitable size worms would still go through the second fences (Fig.2).
The second plan was the C. elegans’ synchronization.[1](How to do the C. elegans’ synchronization ) We got the embryos (Fig.3) from the old worms so that the worms would be at the same stage because of the hatches of the embryos were at the same time. We selected several conditions of the synchronization, finally, we could get the worms at the same stage. The synchronous rate: {{SUSTech_Shenzhen/bmath|equ=
N1 equals the number of worms at L4
N2 equals the number of all worms.
could reach to about 80%.
The Gaussian Plate
The Gaussian Plate (Fig.4)[2] was designed to test if our exogenous genes would influence their olfactory receptor neuron pair (preference and repulsion to some chemical odors).
We got the worms’ distributions(Fig.5) after several experiments for the wild type worms and our experimental worms with or without the chemicals (Fig.6).
The final result were not such a good Gaussian distribution like the Galton board because the C. elegans' choices were not absolutely normal. In order to adjust our results we built a model.(the worm locomotion model)
The Immobilization Chip
The immobilization Chip was deigned to immobilize the C. elegans in worm traps or parallel channels for worm imaging and ethological experiments.[3]
We could immobilize the worms in the worm traps (Fig.8) and watch the neuronal activity (Fig.9) successfully using fluorescence microscope (Nikon eclipse Ti).
- ↑ Portadelariva, M., Fontrodona, L., Villanueva, A., & Cerón, J. (2012). Basic caenorhabditis elegans methods: synchronization and observation. Journal of Visualized Experiments Jove, 64(64), e4019.
- ↑ Albrecht, D. R. and C. I. Bargmann (2011). “High-content behavioral analysis of Caenorhabditis elegans in precise spatiotemporal chemical environments.” Nature Methods 8(7): 599-605.
- ↑ San-Miguel, A., & Lu, H. (2013). Microfluidics as a tool for C. elegans research. Wormbook the Online Review of C Elegans Biology, 1.