We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single system. Our favorite composite part is TRE-Tri_display-hGH (K2520029). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).

			Name	Type	Description	Designer	Length
			BBa_K2520003	Device	TRE-mono display:pro insulin-hGH	Noa Eden, Dana Kadosh	1919
			BBa_K2520004	Device	TRE-mono display:MOG1-hGH	Noa Eden, Dana Kadosh	1139
			BBa_K2520005	Device	TRE-mono display:MOG 3-hGH	Dana Kadosh, Noa Eden	1106
			BBa_K2520006	Device	TRE-GFP-hGH	Noa Eden, Dana Kadosh, Maya Engal	1532
favorite			BBa_K2520007	Device	TRE-Tri display-hGH	Dana Kadosh, Noa Eden	2057
			BBa_K2520008	Device	CMV-Tri display-hGH	Dana Kadosh, Noa Eden	2358
			BBa_K2520009	Device	CMV-tTA-hGH	Noa Eden, Dana Kadosh, Maya Engal	1860
			BBa_K2520010	Device	EF1a-Tri display-hGH	Dana Kadosh, Noa Eden	2994
			BBa_K2520011	Device	EF1a-GFP-hGH	Dana Kadosh, Noa Eden	2469
			BBa_K2520012	Device	EF1a promoter-mono pro insulin-hGH	Dana Kadosh, Noa Eden	2856
			BBa_K2520013	Device	CMV-mono display:MOG 1-hGH	Noa Eden, Dana Kadosh	1511
			BBa_K2520014	Device	CMV-mono display:MOG 3-hGH	Dana Kadosh, Noa Eden	1407
			BBa_K2520015	Device	CMV-Mono Display:celiac 1-hGH	Dana Kadosh, Noa Eden	1475
			BBa_K2520016	Device	CMV-Mono Display:celiac 3-hGHH	Dana Kadosh, Noa Eden	1475
			BBa_K2520017	Device	CMV-mono Display:PLA 1-hGH	Noa Eden, Dana Kadosh	1484
			BBa_K2520018	Device	CMV-Mono Display:PLA 3-hGH	Dana Kadosh, Noa Eden	1502
			BBa_K2520019	Device	CMV-Mono Display:peanut 1-hGH	Dana Kadosh, Noa Eden	1466
			BBa_K2520020	Device	CMV-Mono Display:peanut 3-hGH	Dana Kadosh, Noa Eden	1466