Part Improvements

We have improved two existing parts in an attempt to increase their utility to the iGEM community.
We improved the existing part (BBa_K1119008) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. By replacing the CMV promoter with TRE promoter (BBa_K2520006) we have created a convenient and important positive control for TRE based inducible systems.

Additionally, in our project we used the existing tTA transactivator part (BBa_K1061015) to test our Delay Mechanism. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort.
In order to save future teams time, we have improved this part (BBa_K2520009) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it.

			Name	Type	Description	Designer	Length
			BBa_K2520006	Device	TRE-GFP-hGH	Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia	1532
			BBa_K2520009	Device	CMV-tTA-hGH	Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia	1860

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Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

igem.technion.2017@gmail.com

Team:TECHNION-ISRAEL/Improve

Part Improvements