Composite Parts

We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single plasmid vector.

Our favorite composite part is TRE-Tri display-hGH (BBa_K2520007). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).

	Name	Type	Description	Designer	Length
	BBa_K2520003	Device	TRE-mono display:pro insulin-hGH	Noa Eden, Dana Kadosh	1919
	BBa_K2520004	Device	TRE-mono display:MOG1-hGH	Noa Eden, Dana Kadosh	1139
	BBa_K2520005	Device	TRE-mono display:MOG 3-hGH	Dana Kadosh, Noa Eden	1106
	BBa_K2520006	Device	TRE-GFP-hGH	Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia	1532
favorite	BBa_K2520007	Device	TRE-Tri display-hGH	Dana Kadosh, Noa Eden	2057
	BBa_K2520008	Device	CMV-Tri display-hGH	Dana Kadosh, Noa Eden	2358
	BBa_K2520009	Device	CMV-tTA-hGH	Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia	1860
	BBa_K2520010	Device	EF1a-Tri display-hGH	Dana Kadosh, Noa Eden	2994
	BBa_K2520011	Device	EF1a-GFP-hGH	Dana Kadosh, Noa Eden	2469
	BBa_K2520012	Device	EF1a promoter-mono pro insulin-hGH	Dana Kadosh, Noa Eden	2856
	BBa_K2520013	Device	CMV-mono display:MOG 1-hGH	Noa Eden, Dana Kadosh	1511
	BBa_K2520014	Device	CMV-mono display:MOG 3-hGH	Dana Kadosh, Noa Eden	1407
	BBa_K2520015	Device	CMV-Mono Display:celiac 1-hGH	Dana Kadosh, Noa Eden	1475
	BBa_K2520016	Device	CMV-Mono Display:celiac 3-hGHH	Dana Kadosh, Noa Eden	1475
	BBa_K2520017	Device	CMV-mono Display:PLA 1-hGH	Noa Eden, Dana Kadosh	1484
	BBa_K2520018	Device	CMV-Mono Display:PLA 3-hGH	Dana Kadosh, Noa Eden	1502
	BBa_K2520019	Device	CMV-Mono Display:peanut 1-hGH	Dana Kadosh, Noa Eden	1466
	BBa_K2520020	Device	CMV-Mono Display:peanut 3-hGH	Dana Kadosh, Noa Eden	1466