Part Collection

Meet our "Optimized Mammalian Display Collection"- the collection that will make displaying proteins on the cellular membrane of mammalian cells easy and efficient. During our project we optimized our own membrane display system, and as a result, we created three new basic parts and multiple devices to allow for the display of any recombinant protein future iGEM teams may want.
Within our collection you will find modular plasmids that can display up to three different proteins on the cellular membrane, and in equimolar levels. These plasmids have been submitted with multiple promoters- an inducible promoter and strong mammalian promoters, in order to allow for more versatile and application specific uses (figure 1). You can also find plasmids that constrictively, or inductively, express the GFP reporter gene for positive controls and transfection efficiency quantification.

Figure 1: You can choose between 3 different promoters to achieve both a constitutive and an inducible expression.

As a part of our collection, we submitted three new basic parts for strong and consistent display of proteins on the membrane- Mutated EF1a promoter (BBa_K2520023) for strong mammalian expression, Secrecon (BBa_K2520002)- the most powerful and consistent human secretion signal sequence (figure 2) and B7-1 (BBa_K2520000), a trans-membrane domain that anchors proteins to the membrane with tremendous efficiency (figure 3).

Figure 2: You can change the secretion signal sequence from IgK-leader to Secrecon.

Figure 3: You can change the trans-membrane domain from PDGFR to B7-1.

We believe that this internally complete collection will be easy to use, and beneficial to future iGEM teams.

	Name	Type	Description	Designer	Length
	BBa_K2520000	Protein_Domain	B7-1 Murine Trans Membrane Domain	Dana Kadosh, Noa Eden	213
	BBa_K2520002	Coding	Secrecon	Noa Eden, Dana Kadosh	699
	BBa_K2520003	Device	TRE-mono display:pro insulin-hGH	Noa Eden, Dana Kadosh	1919
	BBa_K2520006	Device	TRE-GFP-hGH	Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia	1532
favorite	BBa_K2520007	Device	TRE-Tri display-hGH	Dana Kadosh, Noa Eden	2057
	BBa_K2520008	Device	CMV-Tri display-hGH	Dana Kadosh, Noa Eden	2358
	BBa_K2520009	Device	CMV-tTA-hGH	Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia	1860
	BBa_K2520010	Device	EF1a-Tri display-hGH	Dana Kadosh, Noa Eden	2994
	BBa_K2520011	Device	EF1a-GFP-hGH	Dana Kadosh, Noa Eden	2469
	BBa_K2520012	Device	EF1a promoter-mono pro insulin-hGH	Dana Kadosh, Noa Eden	2856