Difference between revisions of "Team:TECHNION-ISRAEL/Composite Part"

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{{TECHNION-ISRAEL}}
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<html lang="en">
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<head>
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<!-- Title for the page & metadata  -->
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<title>Composite parts</title>
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<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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<script>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<body>
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<br>
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<div class = "container-fluid">
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<div class= "row">
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<h1> Composite Parts </h1>
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<img src="https://static.igem.org/mediawiki/2017/2/26/T--TECHNION-ISRAEL--part_composite.png" class="cover" alt=""  style= "width:18% ; margin: auto;">
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<div class="row">
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<div class =  "col-md-12" >
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<br>
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<p>
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We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single plasmid vector.
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<br>
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<br>
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<strong>Our favorite composite part is TRE-Tri display-hGH </strong>(<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007">BBa_K2520007</a>). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).
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<br>
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<br>
  
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<div class="column full_size">
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    <table class="table table-list-search">
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<h1>Composite Parts</h1>
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                    <thead>
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                        <tr>
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                            <th> </th>
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                            <th> </th>
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                            <th> </th>
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                            <th>Name</th>
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                            <th>Type</th>
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                            <th>Description</th>
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                            <th>Designer</th>
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                            <th>Length</th>
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                        </tr>
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                    </thead>
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                    <tbody>
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                        <tr>
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<td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520003" target="_blank">BBa_K2520003</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-mono display:pro insulin-hGH</td>
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                            <td>Noa Eden, Dana Kadosh</td>
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<td>1919</td>
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                        </tr>
  
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<p>
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                        <tr>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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                          <td></td>
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</p>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520004" target="_blank">BBa_K2520004</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-mono display:MOG1-hGH</td>
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                            <td>Noa Eden, Dana Kadosh</td>
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<td>1139</td>
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                        </tr>
  
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520005" target="_blank">BBa_K2520005</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-mono display:MOG 3-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>1106</td>
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                        </tr>
  
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<br>
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                        <tr>
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<h3>Best Composite Part Special Prize</h3>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-GFP-hGH</td>
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                            <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td>
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<td>1532</td>
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                        </tr>
  
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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                        <tr>
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                            <td>favorite</td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007" target="_blank">BBa_K2520007</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2057</td>
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                        </tr>
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  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520008" target="_blank">BBa_K2520008</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2358</td>
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                        </tr>
  
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<br><br>
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                        <tr>
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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                            <td></td>
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<br>
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                            <td></td>
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<div class="highlight">
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                            <td></td>
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<h4>Note</h4>
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                            <td>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-tTA-hGH</td>
 +
                            <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td>
 +
<td>1860</td>
 +
                        </tr>
 +
 
 +
                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
 +
                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520010" target="_blank">BBa_K2520010</a>
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</td>
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                            <td>Device</td>
 +
                            <td>EF1a-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>2994</td>
 +
                        </tr>
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 +
 +
 +
 +
<tr>
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                            <td></td>
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                            <td></td>
 +
                            <td></td>
 +
                            <td>
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520011" target="_blank">BBa_K2520011</a>
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</td>
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                            <td>Device</td>
 +
                            <td>EF1a-GFP-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>2469</td>
 +
                        </tr>
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 +
 +
  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520012" target="_blank">BBa_K2520012</a>
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</td>
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                            <td>Device</td>
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                            <td>EF1a promoter-mono pro insulin-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2856</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520013" target="_blank">BBa_K2520013</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-mono display:MOG 1-hGH</td>
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                            <td>Noa Eden, Dana Kadosh</td>
 +
<td>1511</td>
 +
                        </tr>
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 +
                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520014" target="_blank">BBa_K2520014</a>
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</td>
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                            <td>Device</td>
 +
                            <td>CMV-mono display:MOG 3-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>1407</td>
 +
                        </tr>
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 +
<tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520015" target="_blank">BBa_K2520015</a>
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</td>
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                            <td>Device</td>
 +
                            <td>CMV-Mono Display:celiac 1-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
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<td>1475</td>
 +
                        </tr>
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 +
 +
  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520016" target="_blank">BBa_K2520016</a>
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</td>
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                            <td>Device</td>
 +
                            <td>CMV-Mono Display:celiac 3-hGHH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>1475</td>
 +
                        </tr>
 +
 
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520017" target="_blank">BBa_K2520017</a>
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</td>
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                            <td>Device</td>
 +
                            <td>CMV-mono Display:PLA 1-hGH</td>
 +
                            <td>Noa Eden, Dana Kadosh</td>
 +
<td>1484</td>
 +
                        </tr>
 +
 
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520018" target="_blank">BBa_K2520018</a>
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</td>
 +
                            <td>Device</td>
 +
                            <td>CMV-Mono Display:PLA 3-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>1502</td>
 +
                        </tr>
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 +
 +
 +
<tr>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td>
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520019" target="_blank">BBa_K2520019</a>
 +
</td>
 +
                            <td>Device</td>
 +
                            <td>CMV-Mono Display:peanut 1-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>1466</td>
 +
                        </tr>
 +
 +
 +
 +
  <tr>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td>
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520020" target="_blank">BBa_K2520020</a>
 +
</td>
 +
                            <td>Device</td>
 +
                            <td>CMV-Mono Display:peanut 3-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>1466</td>
 +
                        </tr>
 +
 
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                    </tbody>
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                </table> 
  
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</div>
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</div>
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</div>
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</div>
  
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</div>
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Latest revision as of 02:24, 2 November 2017

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Composite parts

Composite Parts




We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single plasmid vector.

Our favorite composite part is TRE-Tri display-hGH (BBa_K2520007). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).

Name Type Description Designer Length
BBa_K2520003 Device TRE-mono display:pro insulin-hGH Noa Eden, Dana Kadosh 1919
BBa_K2520004 Device TRE-mono display:MOG1-hGH Noa Eden, Dana Kadosh 1139
BBa_K2520005 Device TRE-mono display:MOG 3-hGH Dana Kadosh, Noa Eden 1106
BBa_K2520006 Device TRE-GFP-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1532
favorite BBa_K2520007 Device TRE-Tri display-hGH Dana Kadosh, Noa Eden 2057
BBa_K2520008 Device CMV-Tri display-hGH Dana Kadosh, Noa Eden 2358
BBa_K2520009 Device CMV-tTA-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1860
BBa_K2520010 Device EF1a-Tri display-hGH Dana Kadosh, Noa Eden 2994
BBa_K2520011 Device EF1a-GFP-hGH Dana Kadosh, Noa Eden 2469
BBa_K2520012 Device EF1a promoter-mono pro insulin-hGH Dana Kadosh, Noa Eden 2856
BBa_K2520013 Device CMV-mono display:MOG 1-hGH Noa Eden, Dana Kadosh 1511
BBa_K2520014 Device CMV-mono display:MOG 3-hGH Dana Kadosh, Noa Eden 1407
BBa_K2520015 Device CMV-Mono Display:celiac 1-hGH Dana Kadosh, Noa Eden 1475
BBa_K2520016 Device CMV-Mono Display:celiac 3-hGHH Dana Kadosh, Noa Eden 1475
BBa_K2520017 Device CMV-mono Display:PLA 1-hGH Noa Eden, Dana Kadosh 1484
BBa_K2520018 Device CMV-Mono Display:PLA 3-hGH Dana Kadosh, Noa Eden 1502
BBa_K2520019 Device CMV-Mono Display:peanut 1-hGH Dana Kadosh, Noa Eden 1466
BBa_K2520020 Device CMV-Mono Display:peanut 3-hGH Dana Kadosh, Noa Eden 1466
My First Website

Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com