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− | <h1>Composite Parts</h1> | + | |
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+ | <body> | ||
+ | <br> | ||
+ | <div class = "container-fluid"> | ||
+ | <div class= "row"> | ||
+ | |||
+ | |||
+ | <h1> Composite Parts </h1> | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2017/2/26/T--TECHNION-ISRAEL--part_composite.png" class="cover" alt="" style= "width:18% ; margin: auto;"> | ||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <div class = "col-md-offset-2 col-md-8" > | ||
+ | <div class="row"> | ||
+ | <div class = "col-md-12" > | ||
+ | |||
+ | |||
+ | <br> | ||
+ | <p> | ||
+ | We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single plasmid vector. | ||
+ | <br> | ||
+ | <br> | ||
+ | <strong>Our favorite composite part is TRE-Tri display-hGH </strong>(<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007">BBa_K2520007</a>). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta). | ||
+ | <br> | ||
+ | <br> | ||
+ | <table class="table table-list-search"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th> </th> | ||
+ | <th> </th> | ||
+ | <th> </th> | ||
+ | <th>Name</th> | ||
+ | <th>Type</th> | ||
+ | <th>Description</th> | ||
+ | <th>Designer</th> | ||
+ | <th>Length</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | |||
− | < | + | <tr> |
− | + | <td></td> | |
− | </ | + | <td></td> |
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520003" target="_blank">BBa_K2520003</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>TRE-mono display:pro insulin-hGH</td> | ||
+ | <td>Noa Eden, Dana Kadosh</td> | ||
+ | <td>1919</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520004" target="_blank">BBa_K2520004</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>TRE-mono display:MOG1-hGH</td> | ||
+ | <td>Noa Eden, Dana Kadosh</td> | ||
+ | <td>1139</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | < | + | <td></td> |
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520005" target="_blank">BBa_K2520005</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>TRE-mono display:MOG 3-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>1106</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>TRE-GFP-hGH</td> | ||
+ | <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td> | ||
+ | <td>1532</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | < | + | <td>favorite</td> |
− | < | + | <td></td> |
− | < | + | <td></td> |
− | < | + | <td> |
− | < | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007" target="_blank">BBa_K2520007</a> |
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>TRE-Tri display-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>2057</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520008" target="_blank">BBa_K2520008</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-Tri display-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>2358</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-tTA-hGH</td> | ||
+ | <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td> | ||
+ | <td>1860</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520010" target="_blank">BBa_K2520010</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>EF1a-Tri display-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>2994</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520011" target="_blank">BBa_K2520011</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>EF1a-GFP-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>2469</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520012" target="_blank">BBa_K2520012</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>EF1a promoter-mono pro insulin-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>2856</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520013" target="_blank">BBa_K2520013</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-mono display:MOG 1-hGH</td> | ||
+ | <td>Noa Eden, Dana Kadosh</td> | ||
+ | <td>1511</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520014" target="_blank">BBa_K2520014</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-mono display:MOG 3-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>1407</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520015" target="_blank">BBa_K2520015</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-Mono Display:celiac 1-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>1475</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520016" target="_blank">BBa_K2520016</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-Mono Display:celiac 3-hGHH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>1475</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520017" target="_blank">BBa_K2520017</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-mono Display:PLA 1-hGH</td> | ||
+ | <td>Noa Eden, Dana Kadosh</td> | ||
+ | <td>1484</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520018" target="_blank">BBa_K2520018</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-Mono Display:PLA 3-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>1502</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520019" target="_blank">BBa_K2520019</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-Mono Display:peanut 1-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>1466</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520020" target="_blank">BBa_K2520020</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-Mono Display:peanut 3-hGH</td> | ||
+ | <td>Dana Kadosh, Noa Eden</td> | ||
+ | <td>1466</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
− | </ | + | </tbody> |
+ | </table> | ||
− | </div> | + | |
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | <a id="back-to-top" href="#" class="btn btn-lg back-to-top" role="button"><img src="https://static.igem.org/mediawiki/2017/f/f9/T--TECHNION-ISRAEL--newUpAB.png" alt=""></a> | ||
+ | </body> | ||
</html> | </html> | ||
+ | {{:Team:TECHNION-ISRAEL/sponsors}} |
Latest revision as of 02:24, 2 November 2017
Composite Parts
We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single plasmid vector.
Our favorite composite part is TRE-Tri display-hGH (BBa_K2520007). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).
Name | Type | Description | Designer | Length | |||
---|---|---|---|---|---|---|---|
BBa_K2520003 | Device | TRE-mono display:pro insulin-hGH | Noa Eden, Dana Kadosh | 1919 | |||
BBa_K2520004 | Device | TRE-mono display:MOG1-hGH | Noa Eden, Dana Kadosh | 1139 | |||
BBa_K2520005 | Device | TRE-mono display:MOG 3-hGH | Dana Kadosh, Noa Eden | 1106 | |||
BBa_K2520006 | Device | TRE-GFP-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1532 | |||
favorite | BBa_K2520007 | Device | TRE-Tri display-hGH | Dana Kadosh, Noa Eden | 2057 | ||
BBa_K2520008 | Device | CMV-Tri display-hGH | Dana Kadosh, Noa Eden | 2358 | |||
BBa_K2520009 | Device | CMV-tTA-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1860 | |||
BBa_K2520010 | Device | EF1a-Tri display-hGH | Dana Kadosh, Noa Eden | 2994 | |||
BBa_K2520011 | Device | EF1a-GFP-hGH | Dana Kadosh, Noa Eden | 2469 | |||
BBa_K2520012 | Device | EF1a promoter-mono pro insulin-hGH | Dana Kadosh, Noa Eden | 2856 | |||
BBa_K2520013 | Device | CMV-mono display:MOG 1-hGH | Noa Eden, Dana Kadosh | 1511 | |||
BBa_K2520014 | Device | CMV-mono display:MOG 3-hGH | Dana Kadosh, Noa Eden | 1407 | |||
BBa_K2520015 | Device | CMV-Mono Display:celiac 1-hGH | Dana Kadosh, Noa Eden | 1475 | |||
BBa_K2520016 | Device | CMV-Mono Display:celiac 3-hGHH | Dana Kadosh, Noa Eden | 1475 | |||
BBa_K2520017 | Device | CMV-mono Display:PLA 1-hGH | Noa Eden, Dana Kadosh | 1484 | |||
BBa_K2520018 | Device | CMV-Mono Display:PLA 3-hGH | Dana Kadosh, Noa Eden | 1502 | |||
BBa_K2520019 | Device | CMV-Mono Display:peanut 1-hGH | Dana Kadosh, Noa Eden | 1466 | |||
BBa_K2520020 | Device | CMV-Mono Display:peanut 3-hGH | Dana Kadosh, Noa Eden | 1466 |