Difference between revisions of "Team:TECHNION-ISRAEL/Composite Part"

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<h1> Composite Parts </h1>
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<img src="https://static.igem.org/mediawiki/2017/2/26/T--TECHNION-ISRAEL--part_composite.png" class="cover" alt=""  style= "width:18% ; margin: auto;">
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We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single plasmid vector.
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<strong>Our favorite composite part is TRE-Tri display-hGH </strong>(<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007">BBa_K2520007</a>). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).
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                            <th> </th>
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                            <th> </th>
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                            <th> </th>
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                            <th>Name</th>
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                            <th>Type</th>
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                            <th>Description</th>
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                            <th>Designer</th>
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                            <th>Length</th>
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                        </tr>
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                    </thead>
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                    <tbody>
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                        <tr>
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<td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520003" target="_blank">BBa_K2520003</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-mono display:pro insulin-hGH</td>
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                            <td>Noa Eden, Dana Kadosh</td>
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<td>1919</td>
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                        </tr>
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                        <tr>
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                          <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520004" target="_blank">BBa_K2520004</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-mono display:MOG1-hGH</td>
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                            <td>Noa Eden, Dana Kadosh</td>
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<td>1139</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520005" target="_blank">BBa_K2520005</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-mono display:MOG 3-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>1106</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-GFP-hGH</td>
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                            <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td>
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<td>1532</td>
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                        </tr>
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                        <tr>
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                            <td>favorite</td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007" target="_blank">BBa_K2520007</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2057</td>
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                        </tr>
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  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520008" target="_blank">BBa_K2520008</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2358</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-tTA-hGH</td>
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                            <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td>
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<td>1860</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520010" target="_blank">BBa_K2520010</a>
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</td>
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                            <td>Device</td>
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                            <td>EF1a-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2994</td>
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                        </tr>
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 +
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<tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520011" target="_blank">BBa_K2520011</a>
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</td>
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                            <td>Device</td>
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                            <td>EF1a-GFP-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2469</td>
 +
                        </tr>
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  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520012" target="_blank">BBa_K2520012</a>
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</td>
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                            <td>Device</td>
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                            <td>EF1a promoter-mono pro insulin-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2856</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520013" target="_blank">BBa_K2520013</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-mono display:MOG 1-hGH</td>
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                            <td>Noa Eden, Dana Kadosh</td>
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<td>1511</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520014" target="_blank">BBa_K2520014</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-mono display:MOG 3-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>1407</td>
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                        </tr>
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<tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520015" target="_blank">BBa_K2520015</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-Mono Display:celiac 1-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>1475</td>
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                        </tr>
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  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520016" target="_blank">BBa_K2520016</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-Mono Display:celiac 3-hGHH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>1475</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520017" target="_blank">BBa_K2520017</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-mono Display:PLA 1-hGH</td>
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                            <td>Noa Eden, Dana Kadosh</td>
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<td>1484</td>
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                        </tr>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520018" target="_blank">BBa_K2520018</a>
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</td>
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                            <td>Device</td>
 +
                            <td>CMV-Mono Display:PLA 3-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>1502</td>
 +
                        </tr>
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<tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520019" target="_blank">BBa_K2520019</a>
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</td>
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                            <td>Device</td>
 +
                            <td>CMV-Mono Display:peanut 1-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>1466</td>
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                        </tr>
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  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520020" target="_blank">BBa_K2520020</a>
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</td>
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                            <td>Device</td>
 +
                            <td>CMV-Mono Display:peanut 3-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>1466</td>
 +
                        </tr>
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                    </tbody>
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{{:Team:TECHNION-ISRAEL/sponsors}}

Latest revision as of 02:24, 2 November 2017

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Composite parts

Composite Parts




We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single plasmid vector.

Our favorite composite part is TRE-Tri display-hGH (BBa_K2520007). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).

Name Type Description Designer Length
BBa_K2520003 Device TRE-mono display:pro insulin-hGH Noa Eden, Dana Kadosh 1919
BBa_K2520004 Device TRE-mono display:MOG1-hGH Noa Eden, Dana Kadosh 1139
BBa_K2520005 Device TRE-mono display:MOG 3-hGH Dana Kadosh, Noa Eden 1106
BBa_K2520006 Device TRE-GFP-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1532
favorite BBa_K2520007 Device TRE-Tri display-hGH Dana Kadosh, Noa Eden 2057
BBa_K2520008 Device CMV-Tri display-hGH Dana Kadosh, Noa Eden 2358
BBa_K2520009 Device CMV-tTA-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1860
BBa_K2520010 Device EF1a-Tri display-hGH Dana Kadosh, Noa Eden 2994
BBa_K2520011 Device EF1a-GFP-hGH Dana Kadosh, Noa Eden 2469
BBa_K2520012 Device EF1a promoter-mono pro insulin-hGH Dana Kadosh, Noa Eden 2856
BBa_K2520013 Device CMV-mono display:MOG 1-hGH Noa Eden, Dana Kadosh 1511
BBa_K2520014 Device CMV-mono display:MOG 3-hGH Dana Kadosh, Noa Eden 1407
BBa_K2520015 Device CMV-Mono Display:celiac 1-hGH Dana Kadosh, Noa Eden 1475
BBa_K2520016 Device CMV-Mono Display:celiac 3-hGHH Dana Kadosh, Noa Eden 1475
BBa_K2520017 Device CMV-mono Display:PLA 1-hGH Noa Eden, Dana Kadosh 1484
BBa_K2520018 Device CMV-Mono Display:PLA 3-hGH Dana Kadosh, Noa Eden 1502
BBa_K2520019 Device CMV-Mono Display:peanut 1-hGH Dana Kadosh, Noa Eden 1466
BBa_K2520020 Device CMV-Mono Display:peanut 3-hGH Dana Kadosh, Noa Eden 1466
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Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com