Difference between revisions of "Team:TECHNION-ISRAEL/Composite Part"
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| − | <strong>Our favorite composite part is TRE-Tri display-hGH </strong>(<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007">BBa_K2520007</a>). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta). | + | <strong>Our favorite composite part is TRE-Tri display-hGH </strong>(<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007">BBa_K2520007</a>). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta). |
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520003" target="_blank">BBa_K2520003</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520003" target="_blank">BBa_K2520003</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520004" target="_blank">BBa_K2520004</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520004" target="_blank">BBa_K2520004</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520005" target="_blank">BBa_K2520005</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520005" target="_blank">BBa_K2520005</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007" target="_blank">BBa_K2520007</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007" target="_blank">BBa_K2520007</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520008" target="_blank">BBa_K2520008</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520008" target="_blank">BBa_K2520008</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520010" target="_blank">BBa_K2520010</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520010" target="_blank">BBa_K2520010</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520011" target="_blank">BBa_K2520011</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520011" target="_blank">BBa_K2520011</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520012" target="_blank">BBa_K2520012</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520012" target="_blank">BBa_K2520012</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520013" target="_blank">BBa_K2520013</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520013" target="_blank">BBa_K2520013</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520014" target="_blank">BBa_K2520014</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520014" target="_blank">BBa_K2520014</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520015" target="_blank">BBa_K2520015</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520015" target="_blank">BBa_K2520015</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520016" target="_blank">BBa_K2520016</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520016" target="_blank">BBa_K2520016</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520017" target="_blank">BBa_K2520017</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520017" target="_blank">BBa_K2520017</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520018" target="_blank">BBa_K2520018</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520018" target="_blank">BBa_K2520018</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520019" target="_blank">BBa_K2520019</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520019" target="_blank">BBa_K2520019</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
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<td></td> | <td></td> | ||
<td> | <td> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520020" target="_blank">BBa_K2520020</a> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520020" target="_blank">BBa_K2520020</a> |
</td> | </td> | ||
<td>Device</td> | <td>Device</td> | ||
Latest revision as of 02:24, 2 November 2017
Composite Parts
We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single plasmid vector.
Our favorite composite part is TRE-Tri display-hGH (BBa_K2520007). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy antibody detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).
| Name | Type | Description | Designer | Length | |||
|---|---|---|---|---|---|---|---|
| BBa_K2520003 | Device | TRE-mono display:pro insulin-hGH | Noa Eden, Dana Kadosh | 1919 | |||
| BBa_K2520004 | Device | TRE-mono display:MOG1-hGH | Noa Eden, Dana Kadosh | 1139 | |||
| BBa_K2520005 | Device | TRE-mono display:MOG 3-hGH | Dana Kadosh, Noa Eden | 1106 | |||
| BBa_K2520006 | Device | TRE-GFP-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1532 | |||
| favorite | BBa_K2520007 | Device | TRE-Tri display-hGH | Dana Kadosh, Noa Eden | 2057 | ||
| BBa_K2520008 | Device | CMV-Tri display-hGH | Dana Kadosh, Noa Eden | 2358 | |||
| BBa_K2520009 | Device | CMV-tTA-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1860 | |||
| BBa_K2520010 | Device | EF1a-Tri display-hGH | Dana Kadosh, Noa Eden | 2994 | |||
| BBa_K2520011 | Device | EF1a-GFP-hGH | Dana Kadosh, Noa Eden | 2469 | |||
| BBa_K2520012 | Device | EF1a promoter-mono pro insulin-hGH | Dana Kadosh, Noa Eden | 2856 | |||
| BBa_K2520013 | Device | CMV-mono display:MOG 1-hGH | Noa Eden, Dana Kadosh | 1511 | |||
| BBa_K2520014 | Device | CMV-mono display:MOG 3-hGH | Dana Kadosh, Noa Eden | 1407 | |||
| BBa_K2520015 | Device | CMV-Mono Display:celiac 1-hGH | Dana Kadosh, Noa Eden | 1475 | |||
| BBa_K2520016 | Device | CMV-Mono Display:celiac 3-hGHH | Dana Kadosh, Noa Eden | 1475 | |||
| BBa_K2520017 | Device | CMV-mono Display:PLA 1-hGH | Noa Eden, Dana Kadosh | 1484 | |||
| BBa_K2520018 | Device | CMV-Mono Display:PLA 3-hGH | Dana Kadosh, Noa Eden | 1502 | |||
| BBa_K2520019 | Device | CMV-Mono Display:peanut 1-hGH | Dana Kadosh, Noa Eden | 1466 | |||
| BBa_K2520020 | Device | CMV-Mono Display:peanut 3-hGH | Dana Kadosh, Noa Eden | 1466 |
Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel
igem.technion.2017@gmail.com
