Difference between revisions of "Team:TECHNION-ISRAEL/Improve"
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| − | We have improved two existing parts in an attempt to increase their utility to the iGEM community. | + | We have improved two existing parts in an attempt to increase their utility to the iGEM community. <br> |
| − | We improved the existing part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1119008">BBa_K1119008</a>) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. By replacing the CMV promoter with TRE promoter (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006">BBa_K2520006</a>) we have created a convenient and important positive control for TRE based inducible systems. | + | We improved the existing part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1119008">BBa_K1119008</a>) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. <br>By replacing the CMV promoter with TRE promoter (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006">BBa_K2520006</a>) we have created a convenient and important positive control for TRE based inducible systems. |
Additionally, in our project we used the existing tTA transactivator part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1061015">BBa_K1061015</a>) to test our DELAY MECHANISM. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort. In order to save future teams time, we have improved this part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009">BBa_K2520009</a>) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it. | Additionally, in our project we used the existing tTA transactivator part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1061015">BBa_K1061015</a>) to test our DELAY MECHANISM. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort. In order to save future teams time, we have improved this part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009">BBa_K2520009</a>) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it. | ||
Revision as of 17:46, 30 October 2017
Parts Improvement
We have improved two existing parts in an attempt to increase their utility to the iGEM community.
We improved the existing part (BBa_K1119008) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator.
By replacing the CMV promoter with TRE promoter (BBa_K2520006) we have created a convenient and important positive control for TRE based inducible systems.
Additionally, in our project we used the existing tTA transactivator part (BBa_K1061015) to test our DELAY MECHANISM. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort. In order to save future teams time, we have improved this part (BBa_K2520009) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it.
| Name | Type | Description | Designer | Length | |||
|---|---|---|---|---|---|---|---|
| BBa_K2520006 | Device | TRE-GFP-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1532 | |||
| BBa_K2520009 | Device | CMV-tTA-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1860 |
Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel
igem.technion.2017@gmail.com
