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Revision as of 18:44, 30 October 2017

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part improvements

Part Improvements




We have improved two existing parts in an attempt to increase their utility to the iGEM community.
We improved the existing part (BBa_K1119008) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. By replacing the CMV promoter with TRE promoter (BBa_K2520006) we have created a convenient and important positive control for TRE based inducible systems.

Additionally, in our project we used the existing tTA transactivator part (BBa_K1061015) to test our Delay Mechanism. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort.
In order to save future teams time, we have improved this part (BBa_K2520009) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it.




Name Type Description Designer Length
BBa_K2520006 Device TRE-GFP-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1532
BBa_K2520009 Device CMV-tTA-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1860
My First Website

Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com