Difference between revisions of "Team:TECHNION-ISRAEL/Part Collection"

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<title>Part collection</title>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1> Part Collection </h1>
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<img src="https://static.igem.org/mediawiki/2017/2/20/T--TECHNION-ISRAEL--part_collection.png" class="cover" alt=""  style= "width:18% ; margin: auto;">
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<p>
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Meet our "Optimized Mammalian Display Collection"- the collection that will make displaying proteins on the cellular membrane of mammalian cells easy and efficient. During our project we optimized our own membrane display system, and as a result, we created three new basic parts and multiple devices to allow for the display of any recombinant protein future iGEM teams may want.
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<br>
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Within our collection you will find modular plasmids that can display up to three different proteins on the cellular membrane, and in equimolar levels. These plasmids have been submitted with multiple promoters- an inducible promoter and strong mammalian promoters, in order to allow for more versatile and application specific uses <strong>(figure 1)</strong>. You can also find plasmids that constrictively, or inductively, express the GFP reporter gene for positive controls and transfection efficiency quantification.
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<img class="no-rad" src="https://static.igem.org/mediawiki/2017/a/aa/T--TECHNION-ISRAEL--promotor-switch-coll.gif" alt = "" style= "width: 100%; margin:auto;">
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<p style="text-align:center;"><b>Figure 1:</b> You can choose between 3 different promoters to achieve both a constitutive and an inducible expression.
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As a part of our collection, we submitted three new basic parts for strong and consistent display of proteins on the membrane- Mutated EF1a promoter (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520023">BBa_K2520023</a>) for strong mammalian expression, Secrecon (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520002">BBa_K2520002</a>)- the most powerful and consistent human secretion signal sequence <strong>(figure 2)</strong> and B7-1 (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520000">BBa_K2520000</a>), a trans-membrane domain that anchors proteins to the membrane with tremendous efficiency <strong>(figure 3)</strong>.
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<img class="no-rad" src="https://static.igem.org/mediawiki/2017/e/e3/T--TECHNION-ISRAEL--igk-secrecon-coll.gif" alt = "" style= "width: 85%; margin:auto;">
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<p style="text-align:center;"><b>Figure 2:</b> You can change the secretion signal sequence from IgK-leader to Secrecon.
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<img class="no-rad" src=" https://static.igem.org/mediawiki/2017/a/a8/T--TECHNION-ISRAEL--.PDGFR-coll.gif" alt = "" style= "width: 85%; margin:auto;">
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<p style="text-align:center;"><b>Figure 3:</b> You can change the trans-membrane domain from PDGFR to B7-1. 
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<p>
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We believe that this internally complete collection will be easy to use, and beneficial to future iGEM teams.
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</p>
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</div>
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</div>
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<br>
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<br>
  
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    <table class="table table-list-search">
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                    <thead>
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                        <tr>
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                            <th> </th>
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                            <th> </th>
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                            <th> </th>
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                            <th>Name</th>
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                            <th>Type</th>
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                            <th>Description</th>
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                            <th>Designer</th>
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                            <th>Length</th>
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                        </tr>
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                    </thead>
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                    <tbody>
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520000" target="_blank">BBa_K2520000</a>
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</td>
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                            <td>Protein_Domain</td>
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                            <td>B7-1 Murine Trans Membrane Domain</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>213</td>
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                        </tr>
  
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520002" target="_blank">BBa_K2520002</a>
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</td>
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                            <td>Coding</td>
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                            <td>Secrecon</td>
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                            <td>Noa Eden, Dana Kadosh</td>
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<td>699</td>
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                        </tr>
  
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<div class="column full_size">
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                        <tr>
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<td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520003" target="_blank">BBa_K2520003</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-mono display:pro insulin-hGH</td>
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                            <td>Noa Eden, Dana Kadosh</td>
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<td>1919</td>
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                        </tr>
  
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-GFP-hGH</td>
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                            <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td>
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<td>1532</td>
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                        </tr>
  
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<h1> Part Collection </h1>
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                        <tr>
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                            <td>favorite</td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520007" target="_blank">BBa_K2520007</a>
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</td>
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                            <td>Device</td>
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                            <td>TRE-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2057</td>
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                        </tr>
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  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520008" target="_blank">BBa_K2520008</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2358</td>
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                        </tr>
  
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                        <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a>
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</td>
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                            <td>Device</td>
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                            <td>CMV-tTA-hGH</td>
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                            <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td>
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<td>1860</td>
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                        </tr>
  
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520010" target="_blank">BBa_K2520010</a>
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</td>
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                            <td>Device</td>
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                            <td>EF1a-Tri display-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2994</td>
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                        </tr>
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<tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
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                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520011" target="_blank">BBa_K2520011</a>
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</td>
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                            <td>Device</td>
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                            <td>EF1a-GFP-hGH</td>
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                            <td>Dana Kadosh, Noa Eden</td>
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<td>2469</td>
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                        </tr>
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  <tr>
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                            <td></td>
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                            <td></td>
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                            <td></td>
 +
                            <td>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520012" target="_blank">BBa_K2520012</a>
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</td>
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                            <td>Device</td>
 +
                            <td>EF1a promoter-mono pro insulin-hGH</td>
 +
                            <td>Dana Kadosh, Noa Eden</td>
 +
<td>2856</td>
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                        </tr>
  
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
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</p>
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<h3>Best Part Collection Special Prize</h3>
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<p>Did your team make a lot of great parts? Is there a team that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection or a collection of awesome pigment parts? Tell the judges you should be evaluated for the Best Parts Collection award! To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts.
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<br><br>
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If you have a collection of parts you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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<h4>Note</h4>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<a id="back-to-top" href="#" class="btn btn-lg back-to-top" role="button"><img src="https://static.igem.org/mediawiki/2017/f/f9/T--TECHNION-ISRAEL--newUpAB.png" alt=""></a>
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{{:Team:TECHNION-ISRAEL/sponsors}}

Latest revision as of 02:27, 2 November 2017

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Part collection

Part Collection




Meet our "Optimized Mammalian Display Collection"- the collection that will make displaying proteins on the cellular membrane of mammalian cells easy and efficient. During our project we optimized our own membrane display system, and as a result, we created three new basic parts and multiple devices to allow for the display of any recombinant protein future iGEM teams may want.
Within our collection you will find modular plasmids that can display up to three different proteins on the cellular membrane, and in equimolar levels. These plasmids have been submitted with multiple promoters- an inducible promoter and strong mammalian promoters, in order to allow for more versatile and application specific uses (figure 1). You can also find plasmids that constrictively, or inductively, express the GFP reporter gene for positive controls and transfection efficiency quantification.



Figure 1: You can choose between 3 different promoters to achieve both a constitutive and an inducible expression.



As a part of our collection, we submitted three new basic parts for strong and consistent display of proteins on the membrane- Mutated EF1a promoter (BBa_K2520023) for strong mammalian expression, Secrecon (BBa_K2520002)- the most powerful and consistent human secretion signal sequence (figure 2) and B7-1 (BBa_K2520000), a trans-membrane domain that anchors proteins to the membrane with tremendous efficiency (figure 3).



Figure 2: You can change the secretion signal sequence from IgK-leader to Secrecon.




Figure 3: You can change the trans-membrane domain from PDGFR to B7-1.



We believe that this internally complete collection will be easy to use, and beneficial to future iGEM teams.



Name Type Description Designer Length
BBa_K2520000 Protein_Domain B7-1 Murine Trans Membrane Domain Dana Kadosh, Noa Eden 213
BBa_K2520002 Coding Secrecon Noa Eden, Dana Kadosh 699
BBa_K2520003 Device TRE-mono display:pro insulin-hGH Noa Eden, Dana Kadosh 1919
BBa_K2520006 Device TRE-GFP-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1532
favorite BBa_K2520007 Device TRE-Tri display-hGH Dana Kadosh, Noa Eden 2057
BBa_K2520008 Device CMV-Tri display-hGH Dana Kadosh, Noa Eden 2358
BBa_K2520009 Device CMV-tTA-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1860
BBa_K2520010 Device EF1a-Tri display-hGH Dana Kadosh, Noa Eden 2994
BBa_K2520011 Device EF1a-GFP-hGH Dana Kadosh, Noa Eden 2469
BBa_K2520012 Device EF1a promoter-mono pro insulin-hGH Dana Kadosh, Noa Eden 2856
My First Website

Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com