Difference between revisions of "Team:TECHNION-ISRAEL/cell lines"

 
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<h1> Cell Lines </h1>
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<img src="https://static.igem.org/mediawiki/2017/5/5d/T--TECHNION-ISRAEL--co-cell.png" class="cover" alt="" style="width: 25%;">
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<h6> <strong> Team wiki: </strong> </h6>
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<li> <a class="cell" href="#HPC-7" > Maya is beautiful </a> </li>
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<li> <a class="cell" href="#HPC-7" > Shir is cool </a> </li>
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<li> <a class="cell"  href="#HPC-7" > Nuphar is nice and lovely </a> </li>
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Throughout our project we worked with 4 mammalian cell lines:
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<h1> Cell Line</h1>
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<h4> HEK293 </h4>
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<img src ="https://static.igem.org/mediawiki/2017/7/70/T--TECHNION-ISRAEL--hekimg.jpg" class= "cellr" alt="">
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<p> Human embryonic kidney cells 293</p>
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<p>
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HEK293 are a widely used adherent cell line, derived from human embryonic kidney cells, that was transformed by human adenovirus 5 DNA.<sup id = "cite ref-1 " class ="reference">
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<a href="#ref1" original-title>[1] </a>
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</sup>   We chose these cells one of our two <strong>HSC models</strong> because they are easy to grow in culture, and highly susceptible to chemical transfection.
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</p>
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<p>
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The cells were kindly provided by
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<a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions"> Professor Roee Amit's Laboratory</a>.
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<h3 id="HPC-7" > HPC-7 </h3>
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<p>In an attempt to engage with the public and educate different segments of the population we collaborated with “Beit Hagalgalim”, an association for children with mental and physical disabilities. We decided to organize an educational activity based on the concept of <strong>"tolerance towards yourself"</strong> which focused on teaching<strong> self-acceptance </strong>, an important lesson for children who deal daily with adversity and being “different”.
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</p>
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<br>
 
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<p>The activity was a smashing success and the children cooperated amazingly, sharing their feelings, aspirations, and <strong> daily coping methods </strong> for the difficulties they encounter. The activity really left a mark on the kids and to date we still receive incredibly positive feedback both from the counselors and the children.
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<h4 id="HPC7"> HPC-7 </h4>
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<img src ="https://static.igem.org/mediawiki/2017/0/06/T--TECHNION-ISRAEL--hpc7img.jpg" class= "cellr" alt="">
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<p>HPC-7 cells are immortalized murine hematopoietic progenitor cells. This cell line was created by transfecting murine embryonic stem cells with the LHx2
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gene.<sup id = "cite ref-2 " class ="reference">
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<a href="#ref2" original-title>[2]</a>
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</sup> The differentiating potential of these cells is unclear. While they are considered
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multipotent<sup id = "cite ref-3 " class ="reference">
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<a href="#ref3" original-title>[3] </a>
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</sup><sup id = "cite ref-4 " class ="reference">
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<a href="#ref4" original-title>[4] </a>
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</sup>, it appears that they are actually common myeloid progenitors according to a paper from
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1998.<sup id = "cite ref-5 " class ="reference">
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<a href="#ref5" original-title>[5] </a>
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</sup></p>
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<p>
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The cells are grown in suspension, and are very fragile and difficult to grow and transfect.
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</p>
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<p>
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HPC-7 cells were chosen as our second model cell line because they display many characteristics of hematopoietic stem cells
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<sup id = "cite ref-2 " class ="reference">
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<a href="#ref2" original-title>[2] </a>
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</sup> and serve as a recognized model for HSCs
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<sup id = "cite ref-3 " class ="reference">
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<a href="#ref3" original-title>[3] </a>
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</sup>. The cells were kindly given to us by
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<a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions"> Professor Ben-Zion Levi's Laboratory</a>.
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<img src ="https://static.igem.org/mediawiki/2017/1/11/T--TECHNION-ISRAEL--wehi.jpg" class= "cellr" alt="">
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<h4> WEHI-231 </h4>
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WEHI-231 cells are a murine immature B cell lymphoma line. These suspension cells express the phenotype of immature B cells and display IgM antibodies on their membrane. These features made them perfect for our <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Demonstrate"> proof of concept experiment </a>, thus they served as <strong> B cells model.</strong>
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The cells were kindly provided by <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions">Professor  Doron Melamed's Laboratory</a>.
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<h3 id="HEK-293"> HEK-293</h3>
 
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<p>In an attempt to engage with the public and educate different segments of the population we collaborated with “Beit Hagalgalim”, an association for children with mental and physical disabilities. We decided to organize an educational activity based on the concept of <strong>"tolerance towards yourself"</strong> which focused on teaching<strong> self-acceptance </strong>, an important lesson for children who deal daily with adversity and being “different”.
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<h4> CHO-SCF  </h4>
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Chinese hamster ovary cells are epithelial cells derived from a hamster's ovary.
 
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These adherent cells are known for their ability to secrete proteins into the cell culture supernatant. We utilized this property to create stem cell factor, which needed in order to grow the HPC-7 cells. The cells were kindly given by <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions"> Professor Ben-Zion Levi's Laboratory</a>.
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<p>The activity was a smashing success and the children cooperated amazingly, sharing their feelings, aspirations, and <strong> daily coping methods </strong> for the difficulties they encounter. The activity really left a mark on the kids and to date we still receive incredibly positive feedback both from the counselors and the children.
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<div class= "references">
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<ol>
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<li id="ref1">Graham, F. L., et al. "Characteristics of a human cell line transformed by DNA from human adenovirus type 5.<i>" Journal of General Virology</i> 36.1 (1977): 59-72.</li>
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<br>
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<li id="ref2">Kavanagh, Dean PJ, et al. "Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo." <i>PloS one</i> 8.3 (2013): e59150. </li>
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<li id="ref3">Hardison, Ross C. "Finding partners to play the music of regulation." <i>Blood</i> 127.13 (2016): 1624-1626.</li>
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<li id="ref4">Wilson, Nicola K., et al. "Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide analysis of ten major transcriptional regulators." <i>Cell stem cell</i> 7.4 (2010): 532-544.</li>
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<li id="ref5">Kolterud, Åsa, and Leif Carlsson. "Expression of the LIM‐homeobox gene LH2 generates immortalized steel factor‐dependent multipotent hematopoietic precursors." <i>The EMBO journal</i> 17.19 (1998): 5744-5756.</li>
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<h3 id= "WEHI-231" > WEHI-231</h3>
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<p>In an attempt to engage with the public and educate different segments of the population we collaborated with “Beit Hagalgalim”, an association for children with mental and physical disabilities. We decided to organize an educational activity based on the concept of <strong>"tolerance towards yourself"</strong> which focused on teaching<strong> self-acceptance </strong>, an important lesson for children who deal daily with adversity and being “different”.
+
−
</p>
+
−
<br>
+
 
 
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<p>The activity was a smashing success and the children cooperated amazingly, sharing their feelings, aspirations, and <strong> daily coping methods </strong> for the difficulties they encounter. The activity really left a mark on the kids and to date we still receive incredibly positive feedback both from the counselors and the children.
+
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Latest revision as of 01:40, 2 November 2017

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Cell Lines

Cell Lines





Throughout our project we worked with 4 mammalian cell lines:



HEK293

Human embryonic kidney cells 293

HEK293 are a widely used adherent cell line, derived from human embryonic kidney cells, that was transformed by human adenovirus 5 DNA. [1] We chose these cells one of our two HSC models because they are easy to grow in culture, and highly susceptible to chemical transfection.

The cells were kindly provided by Professor Roee Amit's Laboratory.




HPC-7

HPC-7 cells are immortalized murine hematopoietic progenitor cells. This cell line was created by transfecting murine embryonic stem cells with the LHx2 gene. [2] The differentiating potential of these cells is unclear. While they are considered multipotent [3] [4] , it appears that they are actually common myeloid progenitors according to a paper from 1998. [5]


The cells are grown in suspension, and are very fragile and difficult to grow and transfect.

HPC-7 cells were chosen as our second model cell line because they display many characteristics of hematopoietic stem cells [2] and serve as a recognized model for HSCs [3] . The cells were kindly given to us by Professor Ben-Zion Levi's Laboratory.




WEHI-231

WEHI-231 cells are a murine immature B cell lymphoma line. These suspension cells express the phenotype of immature B cells and display IgM antibodies on their membrane. These features made them perfect for our proof of concept experiment , thus they served as B cells model. The cells were kindly provided by Professor Doron Melamed's Laboratory.




CHO-SCF

Chinese hamster ovary cells are epithelial cells derived from a hamster's ovary.

These adherent cells are known for their ability to secrete proteins into the cell culture supernatant. We utilized this property to create stem cell factor, which needed in order to grow the HPC-7 cells. The cells were kindly given by Professor Ben-Zion Levi's Laboratory.




  1. Graham, F. L., et al. "Characteristics of a human cell line transformed by DNA from human adenovirus type 5." Journal of General Virology 36.1 (1977): 59-72.
  2. Kavanagh, Dean PJ, et al. "Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo." PloS one 8.3 (2013): e59150.
  3. Hardison, Ross C. "Finding partners to play the music of regulation." Blood 127.13 (2016): 1624-1626.
  4. Wilson, Nicola K., et al. "Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide analysis of ten major transcriptional regulators." Cell stem cell 7.4 (2010): 532-544.
  5. Kolterud, Åsa, and Leif Carlsson. "Expression of the LIM‐homeobox gene LH2 generates immortalized steel factor‐dependent multipotent hematopoietic precursors." The EMBO journal 17.19 (1998): 5744-5756.
My First Website

Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com