Difference between revisions of "Team:TECHNION-ISRAEL/cell lines"
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| − | HEK293 are a widely used adherent cell line, derived from human embryonic kidney cells, that was transformed by human adenovirus 5 DNA. | + | HEK293 are a widely used adherent cell line, derived from human embryonic kidney cells, that was transformed by human adenovirus 5 DNA.<sup id = "cite ref-1 " class ="reference"> |
| − | + | ||
<a href="#ref1" original-title>[1] </a> | <a href="#ref1" original-title>[1] </a> | ||
| − | </sup> We chose these cells | + | </sup> We chose these cells one of our two <strong>HSC models</strong> because they are easy to grow in culture, and highly susceptible to chemical transfection. |
</p> | </p> | ||
<p> | <p> | ||
| − | The cells were kindly provided by <a href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions"> | + | The cells were kindly provided by |
| + | <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions"> Professor Roee Amit's Laboratory</a>. | ||
</p> | </p> | ||
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| − | <h4> HPC-7 </h4> | + | <h4 id="HPC7"> HPC-7 </h4> |
<img src ="https://static.igem.org/mediawiki/2017/0/06/T--TECHNION-ISRAEL--hpc7img.jpg" class= "cellr" alt=""> | <img src ="https://static.igem.org/mediawiki/2017/0/06/T--TECHNION-ISRAEL--hpc7img.jpg" class= "cellr" alt=""> | ||
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<a href="#ref2" original-title>[2]</a> | <a href="#ref2" original-title>[2]</a> | ||
</sup> The differentiating potential of these cells is unclear. While they are considered | </sup> The differentiating potential of these cells is unclear. While they are considered | ||
| − | multipotent | + | multipotent<sup id = "cite ref-3 " class ="reference"> |
<a href="#ref3" original-title>[3] </a> | <a href="#ref3" original-title>[3] </a> | ||
</sup><sup id = "cite ref-4 " class ="reference"> | </sup><sup id = "cite ref-4 " class ="reference"> | ||
<a href="#ref4" original-title>[4] </a> | <a href="#ref4" original-title>[4] </a> | ||
| − | </sup> it appears that they are actually common myeloid progenitors according to a paper from | + | </sup>, it appears that they are actually common myeloid progenitors according to a paper from |
1998.<sup id = "cite ref-5 " class ="reference"> | 1998.<sup id = "cite ref-5 " class ="reference"> | ||
<a href="#ref5" original-title>[5] </a> | <a href="#ref5" original-title>[5] </a> | ||
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<sup id = "cite ref-3 " class ="reference"> | <sup id = "cite ref-3 " class ="reference"> | ||
<a href="#ref3" original-title>[3] </a> | <a href="#ref3" original-title>[3] </a> | ||
| − | </sup>. The cells were kindly given to us by <a href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions">Professor Ben-Zion Levi's | + | </sup>. The cells were kindly given to us by |
| + | <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions"> Professor Ben-Zion Levi's Laboratory</a>. | ||
</p> | </p> | ||
<br> | <br> | ||
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<img src ="https://static.igem.org/mediawiki/2017/1/11/T--TECHNION-ISRAEL--wehi.jpg" class= "cellr" alt=""> | <img src ="https://static.igem.org/mediawiki/2017/1/11/T--TECHNION-ISRAEL--wehi.jpg" class= "cellr" alt=""> | ||
| − | <h4> WEHI 231 </h4> | + | <h4> WEHI-231 </h4> |
<p> | <p> | ||
| − | WEHI 231 cells are a murine immature B cell lymphoma line. These suspension cells express the phenotype of immature B cells and display IgM antibodies on their membrane. These features made them perfect for our <a href="https://2017.igem.org/Team:TECHNION-ISRAEL/Demonstrate">proof of concept experiment</a>. The cells were kindly provided by <a href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions">Doron Melamed's | + | WEHI-231 cells are a murine immature B cell lymphoma line. These suspension cells express the phenotype of immature B cells and display IgM antibodies on their membrane. These features made them perfect for our <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Demonstrate"> proof of concept experiment </a>, thus they served as <strong> B cells model.</strong> |
| + | The cells were kindly provided by <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions">Professor Doron Melamed's Laboratory</a>. | ||
</p> | </p> | ||
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| − | These adherent cells are known for their ability to secrete proteins into the cell culture supernatant. We utilized this property to create stem cell factor, which needed in order to grow the HPC-7 cells. The cells were kindly given by <a href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions"> Professor Ben-Zion Levi's | + | These adherent cells are known for their ability to secrete proteins into the cell culture supernatant. We utilized this property to create stem cell factor, which needed in order to grow the HPC-7 cells. The cells were kindly given by <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/Attributions"> Professor Ben-Zion Levi's Laboratory</a>. |
</p> | </p> | ||
<br> | <br> | ||
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<div class= "references"> | <div class= "references"> | ||
<ol> | <ol> | ||
| − | <li id="ref1">Graham | + | <li id="ref1">Graham, F. L., et al. "Characteristics of a human cell line transformed by DNA from human adenovirus type 5.<i>" Journal of General Virology</i> 36.1 (1977): 59-72.</li> |
| − | <li id="ref2"> | + | <li id="ref2">Kavanagh, Dean PJ, et al. "Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo." <i>PloS one</i> 8.3 (2013): e59150. </li> |
| − | <li id="ref3">Ross C. | + | <li id="ref3">Hardison, Ross C. "Finding partners to play the music of regulation." <i>Blood</i> 127.13 (2016): 1624-1626.</li> |
| − | <li id="ref4"> | + | <li id="ref4">Wilson, Nicola K., et al. "Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide analysis of ten major transcriptional regulators." <i>Cell stem cell</i> 7.4 (2010): 532-544.</li> |
| − | <li id="ref5"> | + | <li id="ref5">Kolterud, Åsa, and Leif Carlsson. "Expression of the LIM‐homeobox gene LH2 generates immortalized steel factor‐dependent multipotent hematopoietic precursors." <i>The EMBO journal</i> 17.19 (1998): 5744-5756.</li> |
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Latest revision as of 01:40, 2 November 2017
Cell Lines
Throughout our project we worked with 4 mammalian cell lines:
HEK293
Human embryonic kidney cells 293
HEK293 are a widely used adherent cell line, derived from human embryonic kidney cells, that was transformed by human adenovirus 5 DNA. [1] We chose these cells one of our two HSC models because they are easy to grow in culture, and highly susceptible to chemical transfection.
The cells were kindly provided by Professor Roee Amit's Laboratory.
HPC-7
HPC-7 cells are immortalized murine hematopoietic progenitor cells. This cell line was created by transfecting murine embryonic stem cells with the LHx2 gene. [2] The differentiating potential of these cells is unclear. While they are considered multipotent [3] [4] , it appears that they are actually common myeloid progenitors according to a paper from 1998. [5]
The cells are grown in suspension, and are very fragile and difficult to grow and transfect.
HPC-7 cells were chosen as our second model cell line because they display many characteristics of hematopoietic stem cells [2] and serve as a recognized model for HSCs [3] . The cells were kindly given to us by Professor Ben-Zion Levi's Laboratory.
WEHI-231
WEHI-231 cells are a murine immature B cell lymphoma line. These suspension cells express the phenotype of immature B cells and display IgM antibodies on their membrane. These features made them perfect for our proof of concept experiment , thus they served as B cells model. The cells were kindly provided by Professor Doron Melamed's Laboratory.
CHO-SCF
Chinese hamster ovary cells are epithelial cells derived from a hamster's ovary.
These adherent cells are known for their ability to secrete proteins into the cell culture supernatant. We utilized this property to create stem cell factor, which needed in order to grow the HPC-7 cells. The cells were kindly given by Professor Ben-Zion Levi's Laboratory.
- Graham, F. L., et al. "Characteristics of a human cell line transformed by DNA from human adenovirus type 5." Journal of General Virology 36.1 (1977): 59-72.
- Kavanagh, Dean PJ, et al. "Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo." PloS one 8.3 (2013): e59150.
- Hardison, Ross C. "Finding partners to play the music of regulation." Blood 127.13 (2016): 1624-1626.
- Wilson, Nicola K., et al. "Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide analysis of ten major transcriptional regulators." Cell stem cell 7.4 (2010): 532-544.
- Kolterud, Åsa, and Leif Carlsson. "Expression of the LIM‐homeobox gene LH2 generates immortalized steel factor‐dependent multipotent hematopoietic precursors." The EMBO journal 17.19 (1998): 5744-5756.
Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel
igem.technion.2017@gmail.com
