Team Members

Jianan Li

Qingrui Sun

Yiming Rong

Jiayue Guo

Zhiyuan Lu

Meiqi Yuan

Zhengyao Lin

Caiyi Feng

Shuyun Zhang

Tenghao Huang

Yining Huang

Yanyue Zhu

Protocols

Our practices followed the protocol for plate reader measurement provided by iGEM HQ, except that we used a 2xTY media instead of the LB stated in the protocol.
Standard curve and OD600 reference point were measured before-hand. The machine used was Synergy Neo2.

Parts and Materials

Parts

Positive control

Negative control

Test Device 1: J23101+I13504

Test Device 2: J23106+I13504

Test Device 3: J23117+I13504

Test Device 4: J23101.BCD2.E0040.B0015

Test Device 5: J23106.BCD2.E0040.B0015

Test Device 6: J23117.BCD2.E0040.B0015

Materials

LUDOX-HS-40 Silica x3

Fluorescein Sodium Salt
One tube of dried down fluorescein sodium salt resuspended in 1.0mL PBS
Concentration after resuspension: 100uM

Machine

Synergy Neo2 reader

Results

OD600 Reference Points

	LUDOX-HS40	H2O
Replicate 1	0.046	0.042	Enter Abs600 absorbance measurements into blue cells
Replicate 2	0.049	0.039	Gold cells are calculated
Replicate 3	0.046	0.036
Replicate 4	0.045	0.038
Arith. Mean	0.0465	0.03875
Corrected Abs600	0.00775		Corrected value is particle-only contribution
Reference OD600	0.0425		Reference value is for 100uL of LUDOX-HS40 in a well of a standard 96-well flat-bottom plate
OD600/Abs600	5.48387096774194		Corrected value = scaling factor * measured value

To see the rest of our results, please click here.

Team:HFLS H2Z Hangzhou/InterLab