Team:NIPER-Guwahati/attributions
ATTRIBUTIONS
Department of Biotechnology, Ministry of Science and Technology: Sponsored our project under Indian Biological Engineering Competition (iBEC), a pre-iGEM competition which provides financial support to iGEM teams from India on the basis of potential applications of proposed work.
State Bank of India: They also sponsored our project and provided financial support and funding.
NIPER – Guwahati: The initial fund required for registration in iGEM was provided by our institute. We are also grateful to our institute for providing us funding for labs and the research facility.
Guwahati Biotech Park (GBP): GBP provided us labs, instruments and other facilities. All our work was carried out here.
IIT Guwahati: We are grateful to Dr. Ajaikumar B. Kunnumakkara and the PhD students from the Cancer Biology Laboratory, Department of Biosciences and Bioengineering, IIT - Guwahati for providing us the lab space and facilities we needed for performing RT – PCR for validation of our work.
Integrated DNA Technologies (IDT): IDT is the world's largest supplier of custom nucleic acids, serving the areas of academic research, biotechnology, clinical diagnostics, and pharmaceutical development. It provided us biotin coated BCl-2 mRNA and the primers we required for PCR.
TEAM WORK:
Project Concept was brought forward by our primary PI Dr. Utpal Mohan and advisors Veera Vijay and G. Vijaykumar.
Protocols and project strategy were optimized by Dr. Swapnil Sinha and Dr. Utpal Mohan
Synthesis and selection of DNAzymes specific to BCL-2 were performed by Veera Vijay , Manasa and G. Vijaykumar
Cloning and ligation of inserts into vectors were performed by Arjun Prasad and Shashikant Patil with advice from G. Vijaykumar
Cell culturing of A375 cell line for transfection of DNAzymes was done by our teammates Prajakta Deshmukh, Abiya Johnson and our advisor U. Shravan Kumar.
Transfection of DNAzymes was done by U. Shravan Kumar and Prajakta Deshmukh
Wet Lab work was handled mainly by K. Sireesha along with all the team members
Validation of DNAzymes using RT-PCR was performed by Prajakta Deshmukh, Abiya Johnson and our advisor G. Vijaykumar in the Cancer Biology Laboratory, IIT- Guwahati
Phage surface display approach was performed by Prajakta Deshmukh, Abiya Johnson, Shashikant Patil and Vijay Agarwal to select cell penetrating peptides specific to A375 melanoma cell line
Wiki page Design was handled mainly by Manasa Reddy and Abiya Johnson with the help of our advisor Mrs. Nijara Sarmah.
Wiki Content development: All our team members and team advisors contributed to developing the contents to be placed on our Wiki. This was initiated by Arjun Prasad, Manasa Reddy and Abiya Johnson.
