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<h4>Patch clamp</h4> | <h4>Patch clamp</h4> | ||
<ul> | <ul> | ||
− | <li>We used whole-cell patch clamp to measure the membrane voltage and ion currents across a HEK cell membrane.</li> | + | <li>We used whole-cell patch clamp to measure the membrane voltage and ion currents across a HEK cell membrane. To measure whole-cell, we pushed a glass pipette against the memrane of a cell, creating a complete seal of the membrane against the pipette. Afterwards, we caused a sudden negative pressure in the glass pipette, which destroys the membrane in the opening of the glass pipette. The solution within the pipett will subsequentially mix with the intracellular compartment of the cell, allowing for measuring with a pre-defined intracellular concentration.</li> |
</ul> | </ul> | ||
<h5>Voltage clamp</h5> | <h5>Voltage clamp</h5> |
Revision as of 13:09, 4 September 2017
Protocols
In the lab, we used different experimental procedures. There are protocols for the wet and bacterial lab, for the cell culture lab and for the electrophysiology lab.