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− | < | + | We successfully improve characterization of parts <html><a href="http://parts.igem.org/Part:BBa_K309016">BBa_K309016</a></html>—GFP with introns used in C.elegans. What’s more, we optimize the sequence to make the coding sequence stats with “ATG”. The optimized GFP is submitted. |
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+ | We construct a str-1::Chrimson::GEMgeco::GFP fusion gene and inject it into C.elegans. The function of GFP is to localize the neuron AWB with the help of promoter str-1. | ||
− | + | We capture the image of GFP specifically expressed in AWB with confocal microscope and clearly observe AWB even its dendrite. | |
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− | </ | + | {{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--Parts-Charcterization.png|width=1000px|caption=<B>Head of <i>C.elegans</i>, the arrow point to neuron AWB.</B>}} |
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Revision as of 13:21, 23 October 2017
Parts
Contribution
Contents
Parts Characterization
We successfully improve characterization of parts BBa_K309016—GFP with introns used in C.elegans. What’s more, we optimize the sequence to make the coding sequence stats with “ATG”. The optimized GFP is submitted.
We construct a str-1::Chrimson::GEMgeco::GFP fusion gene and inject it into C.elegans. The function of GFP is to localize the neuron AWB with the help of promoter str-1.
We capture the image of GFP specifically expressed in AWB with confocal microscope and clearly observe AWB even its dendrite.