Difference between revisions of "Team:Austin UTexas/Parts"

Registry Parts

Composite Parts

We submitted two composite PhytoBrick parts to the registry. Each part is described below. The link to each page is provided in bold text.

Constitutive P8 and P32 Lactococcus lactis promoter and RBS composite parts

The P8 and P32 constitutive promoters and RBS sequences are natively found Lactococcus lactis. Although the transcriptional efficiency of these promoters has been characterized and tested in Lactococcus lactis and other Gram-positive bacteria, their functionality in Gram-negative species such as E. coli has not been recorded in the literature. Thus, we assessed the functionality of these promoters in E. coli using the E-2 Crimson reporter gene, which encodes a red fluorescent protein. We have confirmed that E. coli transformed with cassette plasmids containing these P8 and P32 reporter gene constructs are able to successfully express the E2-Crimson red fluorescent protein. As a PhytoBrick compatible part containing the proper overhangs, the P8 promoter can be assembled into a transcriptional unit via Golden Gate assembly method to upregulate expression of a gene of interest in E. coli as well as L. lactis. The PhytoBrick parts for the P8 and P32 promoters can be found by clicking these two links, respectively: [link] and [link].