Difference between revisions of "Team:Lethbridge/Experiments"
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<h2 class="segmentHeader">Protocols</h2> | <h2 class="segmentHeader">Protocols</h2> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf">LB Media</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf">LB Media</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf">Agarose Gel Extraction</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf">Agarose Gel Extraction</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf">PCR Amplification</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf">PCR Amplification</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf">Colony PCR</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf">Colony PCR</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf">DNA PUrification from Enzymatic Reactions</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf">DNA PUrification from Enzymatic Reactions</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf">PCR product Purification</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf">PCR product Purification</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf">In Vitro Transcription</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf">In Vitro Transcription</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/6/6c/Ligation_protocol.pdf">Ligations</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/6/6c/Ligation_protocol.pdf">Ligations</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/d/df/PhenolChloroform_extraction_%26_ethanol_percipitation.pdf">Phenol/Chloroform Extraction and Ethanol Precipitation</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/d/df/PhenolChloroform_extraction_%26_ethanol_percipitation.pdf">Phenol/Chloroform Extraction and Ethanol Precipitation</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf">pJET Cloning</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf">pJET Cloning</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf">Plasmid Miniprep</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf">Plasmid Miniprep</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf">Preparation of Chemical Competent Cells</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf">Preparation of Chemical Competent Cells</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf">Protein Overexpression</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf">Protein Overexpression</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf">PURExpress NEB Kit</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf">PURExpress NEB Kit</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf">Purification of Poly-Histidine Tagged Proteins</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf">Purification of Poly-Histidine Tagged Proteins</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf">Restriction Enzyme Digest and Dephosphorylation</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf">Restriction Enzyme Digest and Dephosphorylation</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf">T7- RNA Polymerase Purification</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf">T7- RNA Polymerase Purification</a></p> | ||
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<p><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf">Transformation</a></p> | <p><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf">Transformation</a></p> | ||
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<br><br><br><br> | <br><br><br><br> | ||
Revision as of 16:42, 28 October 2017
Protocols
DNA PUrification from Enzymatic Reactions
Phenol/Chloroform Extraction and Ethanol Precipitation
Preparation of Chemical Competent Cells
Purification of Poly-Histidine Tagged Proteins
Restriction Enzyme Digest and Dephosphorylation
Products/Reagents
| Enzymes | Buffers/Reagents | Other Products |
|---|---|---|
| Shrimp Alkaline Phosphatase | Affinity Chromatography Buffers | Ni²+ Affinity Resin |
| DNA Blunting Enzyme | IPTG | Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’ |
| Phusion Polymerase Master Mix | Kanomycin/Chloramphenicol/Ampicillin | Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’ |
| PFU Polymerase | Dimethyl Sulfur Dioxide | Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’ |
| T7 RNA Polymerase | Competent Cell Buffers | Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’ |
| T7 DNA Polymerase | T7 RNA Polymerase Purification Buffers | Dialyzation Membrane |
| PURExpressKit (New England Biolabs) | Phenol | |
| XbaI (NEB) | Chloroform | |
| SpeI (NEB) | 10x PFU Buffer | |
| PstI (NEB) | NTP | |
| EcoRI (NEB) | GMP | |
| Dream Taq DNA Polymerase | 10x Taq Buffer | |
| Taq DNA Polymerase | Ligase Buffer | |
| RNAse A | SDS-PAGE Reagents | |
| TraB | Urea PAGE reagents | |
| iPPase | Agarose | |
| RNAse Inhibitor | Agar | |
| DNAseI | Tryptone | |
| Yeast Extract | ||
| TAKMz | BioBasic Plasmid DNA miniprep Kit | |
| Imidazole |
