Difference between revisions of "Team:Hong Kong HKU/Parts"
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<h1>Parts</h1> | <h1>Parts</h1> | ||
| − | This year we produced 5 biobricks each of which produce ssDNA which can come together to form a functional tetrahedron structure in presence of its target. The inserts were ordered as gBlocks fragments from Integrated DNA technologies As shown in the diagram, the insert can be divided into 5 parts: | + | <p>This year we produced 5 biobricks each of which produce ssDNA which can come together to form a functional tetrahedron structure in presence of its target. The inserts were ordered as gBlocks fragments from Integrated DNA technologies As shown in the diagram, the insert can be divided into 5 parts:</p> |
<ol> | <ol> | ||
<li>Biobrick prefix and suffix </li> | <li>Biobrick prefix and suffix </li> | ||
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<li>ssDNA sequence: This sequence is complementary to the ncRNA sequence that will be produced.</li> | <li>ssDNA sequence: This sequence is complementary to the ncRNA sequence that will be produced.</li> | ||
<li>HTBS: HIV reverse transcriptase binding site </li> | <li>HTBS: HIV reverse transcriptase binding site </li> | ||
| − | + | <li>BBa_B0054 : Strong Terminator </li> | |
</ol> | </ol> | ||
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<p> As shown before the Pre-tetra and the Tetra is generated using five nucleotide sequence and we have made a total of 5 biobricks, one biobrick for each. </p> | <p> As shown before the Pre-tetra and the Tetra is generated using five nucleotide sequence and we have made a total of 5 biobricks, one biobrick for each. </p> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/b/ba/Plasmidconstruct1.png" alt="plasmidconstruct" width="800" height="800"> | ||
Revision as of 01:42, 29 October 2017
Parts
This year we produced 5 biobricks each of which produce ssDNA which can come together to form a functional tetrahedron structure in presence of its target. The inserts were ordered as gBlocks fragments from Integrated DNA technologies As shown in the diagram, the insert can be divided into 5 parts:
- Biobrick prefix and suffix
- ProD - This is the Promoter for our biobrick. The promoter allows for high expression of our downstream product
- ssDNA sequence: This sequence is complementary to the ncRNA sequence that will be produced.
- HTBS: HIV reverse transcriptase binding site
- BBa_B0054 : Strong Terminator
We expect that upon transcription a ncRNA will be produced with an HTBS sequence that will allow HIV reverse transcriptase to bind the ncRNA and produce the desired DNA sequence. Upon addition of RNase H and RNase A the DNA: RNA duplex and the degradation can occur respectively.
As shown before the Pre-tetra and the Tetra is generated using five nucleotide sequence and we have made a total of 5 biobricks, one biobrick for each.
<groupparts>iGEM17 Hong_Kong_HKU</groupparts>
