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<h4> Collaboration </h4> | <h4> Collaboration </h4> | ||
<article> | <article> | ||
− | Throughout our project we collaborated with many iGEM Teams to improve our work and help each other. Especially our | + | Throughout our project we collaborated with many iGEM Teams to improve our work and help each other. Especially our part exchange with the <a href="https://2017.igem.org/Team:CU-Boulder">CU Boulder</a> team and the mentoring of the new iGEM team <a href="https://2017.igem.org/Team:UNIFI">UNIFI</a> was very important for us. |
</article> | </article> | ||
<h4> Human Practices </h4> | <h4> Human Practices </h4> |
Revision as of 15:04, 29 October 2017
![](https://static.igem.org/mediawiki/2017/7/74/T--Bielefeld-CeBiTec--title-img-centrifuge.jpg)
Achievements
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Establishment of two orthogonal methods for the detection of unnatural base pairs in a target sequence via Oxford Nanopore sequencing and an enzyme based detection method
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a software suite for these orthogonal methods
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Proof that certain Taq-polymerases can efficiently incorporate unnatural nucleotides
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of a toolkit consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a photoswitchable lycopene pathway
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Design and chemical synthesis of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine and proof of its functionality
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Modeling more than ten new aaRS sequences
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Library development with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and much more
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of an Android App to control the LED panel with your smartphone via Bluetooth
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing of a biosafety report titled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing of the ChImp Report on the “Chances and Implications of an Expanded Genetic Code”
We applied for the following special prizes
Best Integrated Human Practices
Best Education and Public Engagement
Best Measurement
Best Modeling
Best Basic Part
Best Composite Part
Best Part Collection
Best Software Tool
Best Hardware
![](https://static.igem.org/mediawiki/2017/2/28/T--Bielefeld-CeBiTec--YKE_medal_gold_icon.png)
Integrated Human Practices
Improve a previous part or project
Model your project
Demonstrate your work
![](https://static.igem.org/mediawiki/2017/e/e6/T--Bielefeld-CeBiTec--YKE_medal_silver_icon.png)
Validated Part / Validated Contribution
Collaboration
Human Practices
![](https://static.igem.org/mediawiki/2017/5/52/T--Bielefeld-CeBiTec--YKE_medal_bronze_icon.png)