Difference between revisions of "Team:Manchester/Model"
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| − | <img src="https://static.igem.org/mediawiki/2017/8/8d/T--Manchester--DOElogo.jpg"/> | + | <img src="https://static.igem.org/mediawiki/2017/8/8d/T--Manchester--DOElogo.jpg" width="100%"/> |
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<p style="text-align: center" "font-size: 24px!important"><b>Design of Experiments</b></p> | <p style="text-align: center" "font-size: 24px!important"><b>Design of Experiments</b></p> | ||
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| − | <a href="https://2017.igem.org/Team:Manchester/Model/Continuous_Culture"><img src="https://static.igem.org/mediawiki/2017/5/59/T--Manchester--Chemostat_logo.png" /></a> | + | <a href="https://2017.igem.org/Team:Manchester/Model/Continuous_Culture"><img src="https://static.igem.org/mediawiki/2017/5/59/T--Manchester--Chemostat_logo.png" width="100%"/></a> |
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<p style="text-align: center" "font-size: 24px!important"><b>Continuous Culture</b></p> | <p style="text-align: center" "font-size: 24px!important"><b>Continuous Culture</b></p> | ||
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| − | <a href="https://2017.igem.org/Team:Manchester/Model/PSO"><img src="https://static.igem.org/mediawiki/2017/1/10/T--Manchester--Operon_logo.jpg"/></a> | + | <a href="https://2017.igem.org/Team:Manchester/Model/PSO"><img src="https://static.igem.org/mediawiki/2017/1/10/T--Manchester--Operon_logo.jpg" width="100%"/></a> |
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<p style="text-align: center" "font-size: 24px!important"><b>Phosphate Starvation Operon</b></p> | <p style="text-align: center" "font-size: 24px!important"><b>Phosphate Starvation Operon</b></p> | ||
Revision as of 19:48, 29 October 2017
Modelling
1. Continuous culture modelling was used to predict the rate at which Phosphostore devices could be produced on different substrates. This allowed us to estimate the yearly cost of treating wastewater using phosphostore. As a result we performed a major re-design of the intended Phosphostore device, assessing the cost reduction potential of different growth conditions and experimental strategies by computational modelling
2. We used an innovative ensemble modelling approach to predict the operating characteristics of our phosphate starvation operon as a regulatory system for controlling microcompartment synthesis. This helped us choose the appropriate regulatory parts for our experimental design
3. We used statistical Design of Experiments (DoE) to design the most efficient experiments to determine the factors influencing the activity of our key enzyme. Two rounds of DoE enabled us to identify the optimal conditions for testing of our experimental system
Design of Experiments
Continuous Culture
Phosphate Starvation Operon
