Difference between revisions of "Team:Lethbridge/Parts"

The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts (LINK TO PARTS COLLECTION PAGE) that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts (LINK TO IMPROVED PARTS PAGE) for future use.

In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the Next vivo system makes use of a previously submitted MS2BP part (BBA_K1791003) LINK?!?!?!.

Composite Parts

General Protein Construct

General RNA Construct

Contains all 38 proteins essential for prokaryotic transcription and translation (Tx/Tl) as well 3 additional constructs for tRNA purification and Tx/Tl validation.

Composite parts table