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/* PRODUCT REAGENT TABLE ENDS */ | /* PRODUCT REAGENT TABLE ENDS */ | ||
</style> | </style> | ||
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<body> | <body> | ||
<div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png" class="bannerImg"></div> | <div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png" class="bannerImg"></div> | ||
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<td>IPTG</td> | <td>IPTG</td> | ||
<td>Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’</td> | <td>Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>TAKMz</td> | <td>TAKMz</td> | ||
<td> </td> | <td> </td> | ||
− | </tr | + | </tr> |
<tr> | <tr> | ||
<td></td> | <td></td> |
Revision as of 20:06, 29 October 2017
![](https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png)
Protocols
Products/Reagents
Enzymes | Buffers/Reagents | Other Products |
---|---|---|
Shrimp Alkaline Phosphatase | Affinity Chromatography Buffers | Ni²+ Affinity Resin |
DNA Blunting Enzyme | IPTG | Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’ |
Phusion Polymerase Master Mix | Kanomycin / Chloramphenicol / Ampicillin | Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’ |
PFU Polymerase | Dimethyl Sulfur Dioxide | Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’ |
T7 RNA Polymerase | Competent Cell Buffers | Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’ |
T7 DNA Polymerase | T7 RNA Polymerase Purification Buffers | Dialyzation Membrane |
PURExpressKit (New England Biolabs) | Phenol | |
XbaI (NEB) | Chloroform | |
SpeI (NEB) | 10x PFU Buffer | |
PstI (NEB) | NTP | |
EcoRI (NEB) | GMP | |
Dream Taq DNA Polymerase | 10x Taq Buffer | |
Taq DNA Polymerase | Ligase Buffer | |
RNAse A | SDS-PAGE Reagents | |
TraB | Urea PAGE reagents | |
iPPase | Agarose | |
RNAse Inhibitor | Agar | |
DNAseI | Tryptone | |
Yeast Extract | ||
TAKMz | ||
BioBasic Plasmid DNA miniprep Kit | ||
Imidazole |
![](https://static.igem.org/mediawiki/2017/7/7d/Banner_footer_blank.png)