Line 51: | Line 51: | ||
<div style="clear: both"></div> | <div style="clear: both"></div> | ||
− | <p> | + | <p class="text12">This year we designed <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Composite_Part">41 composite parts</a>. Of the 41 parts, it contains 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well 3 additional constructs for tRNA purification and TX-TL validation.</p> |
− | + | ||
</div> | </div> |
Revision as of 20:35, 29 October 2017
The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts for future use.
In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the Next vivo system makes use of a previously submitted MS2BP part (BBA_K2109108).
Part Collection
The Next vivo part collection consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015).
Composite Parts
General Protein Construct
General RNA Construct
This year we designed 41 composite parts. Of the 41 parts, it contains 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well 3 additional constructs for tRNA purification and TX-TL validation.
Basic Parts
All basic parts pages created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (Tx/Tl) as well as the parts for our tRNA purification and Tx/Tl validation construct.