Difference between revisions of "Team:TECHNION-ISRAEL/Experiments"

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<ol class="protocols">
 
<ol class="protocols">
 
<li><a target="_blank" href= "https://static.igem.org/mediawiki/2017/9/93/T--TECHNION-ISRAEL--Transfection_to_HPC-7_with_lipofectamine_2000.pdf">Transfection with Lipofectamine 2000</a></li>
 
<li><a target="_blank" href= "https://static.igem.org/mediawiki/2017/9/93/T--TECHNION-ISRAEL--Transfection_to_HPC-7_with_lipofectamine_2000.pdf">Transfection with Lipofectamine 2000</a></li>
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<li><a target="_blank" href="  https://static.igem.org/mediawiki/2017/1/1c/T--TECHNION-ISRAEL--Splitting_for_HPC-7_cells.pdf">Splitting</a></li>
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<li><a target="_blank" href="  https://static.igem.org/mediawiki/2017/8/89/T--TECHNION-ISRAEL--Splitting_for_HPC-7_cells.PDF">Splitting</a></li>
 
<li><a target="_blank" href="https://static.igem.org/mediawiki/2017/7/74/T--TECHNION-ISRAEL--Multiporator_optimization.pdf">Multiporator Optimization</a></li>
 
<li><a target="_blank" href="https://static.igem.org/mediawiki/2017/7/74/T--TECHNION-ISRAEL--Multiporator_optimization.pdf">Multiporator Optimization</a></li>
 
</ol>
 
</ol>

Revision as of 10:37, 30 October 2017

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Protocols

Protocols



Tissue culture


General

  1. Thawing Cells
  2. Gibco Cell Culture Basics Handbook Global
  1. Freezing Cells




HEK 293

  1. Splitting
  2. Reverse Transfection
  1. Induction Experiment
  2. Medium Recipe


WEHI 231

  1. Medium Recipe


HPC-7

  1. Transfection with Lipofectamine 2000
  2. Splitting
  3. Multiporator Optimization
  1. Transfection with TransIT 2020
  2. Medium Recipe
  3. Electroporation-NEPA21


Interlab


  1. InterLab 2017 Plate Reader


Assays


  1. Extracellular Staining of Adherent Cells
  2. PI Death Assay
  1. Apoptosis Kit
  2. Anti-IgM Apoptosis Assay


Cloning


  1. Cloning Flow Chart
  2. Sequencing (RAMBAM)
  3. Restriction Enzymes
  4. Nanoview
  5. CIP
  6. Primer Dilution
  7. Miniprep
  8. Midiprep
  1. Glycerol Stock
  2. Gibson Assembly
  3. Gel Extraction(to miniprep)
  4. Colony PCR
  5. Chemical Transformation
  6. Agarose Gel Preparation
  7. PCR & revPCR
  8. Gel Elecrophoresis
My First Website

Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com