Difference between revisions of "Team:Austin UTexas/Parts"

Registry Parts

Composite Parts

We submitted two composite PhytoBrick parts and one composite BioBrick part to the registry. Each part is described below. The link to each page is provided in bold text.

Constitutive P8 and P32 Lactococcus lactis promoter and RBS composite parts

The P8 and P32 constitutive promoters and RBS sequences are natively found Lactococcus lactis. Although the transcriptional efficiency of these promoters has been characterized and tested in Lactococcus lactis and other Gram-positive bacteria, their functionality in Gram-negative species such as E. coli has not been recorded in the literature. Thus, we assessed the functionality of these promoters in E. coli using the E-2 Crimson reporter gene, which encodes a red fluorescent protein. We have confirmed that E. coli transformed with cassette plasmids containing these P8 and P32 reporter gene constructs are able to successfully express the E2-Crimson red fluorescent protein. As a PhytoBrick compatible part containing the proper overhangs, the P8 promoter can be assembled into a transcriptional unit via the Golden Gate assembly method to upregulate expression of a gene of interest in E. coli as well as L. lactis.

The constitutive P8 promoter and RBS PhytoBrick composite part can be found on the iGEM registry as: BBa_K2253000. The constitutive P32 promoter and RBS PhytoBrick composite part can be found on the iGEM registry as: BBa_K2253001.