Difference between revisions of "Team:Manchester/Basic Part"

Part Name	BioBrick	Description	Part Type
LacUV5_EutS	BBa_K2213000	The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutS (from E. coli) under control of the LacUV5 inducible promoter. Contains RBS, terminators and all inducible Lac-system parts. EutS is tagged with His6.	Basic
TetR_EutMN	BBa_K2213001	Ethanolamine Utilisation (Eut) bacterial micro-compartment (BMC) proteins EutM and EutN from E. coli, placed under the inducible Tetracycline promoter. Contains RBS, terminators and all tetp components. EutM is tagged with GFP and His6. EutN is tagged with FLAG.	Basic
araBAD_EutLK	BBa_K2213002	The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG.	Basic
cgPPK2_His6	BBa_K2213003	Corynebacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator.	Basic
PduD(1-20)_cgPPK2_mCherry_His6	BBa_K2213004	Corynebacterium PPK2 with a C terminal mCherry-polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator.	Basic
PduD(1-20)_mCherry_cgPPK2_His6	BBa_K2213005	Corynebacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag and mCherry at the N terminal. Contains RBS and terminator.	Basic
LowPromoter_PduD(1-20)_mCherry	BBa_K2213006	BBa_K2213006 with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added.	Basic - Improved
MediumPromoter_PduD(1-20)_mCherry	BBa_K2213007	BBa_K2213006 with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added.	Basic - Improved
HighPromoter_PduD(1-20)_mCherry	BBa_K2213008	BBa_K2213006 with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added.	Basic - Improved
LowPromoter_PduD(1-20)_mCherry_cgPPK2	BBa_K2213009	BBa_K2213006 with cgPPK2 added to the C terminus.	Basic

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