Difference between revisions of "Team:Manchester/Improve"
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<head> | <head> | ||
<style> | <style> | ||
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| − | + | body { | |
| + | margin: 0px!important; | ||
| + | padding: 0px!important | ||
} | } | ||
| − | + | .sectionfirst { | |
| − | + | margin-top: 10px!important; | |
| − | + | padding-top: 10vh; | |
| − | + | height: 30vh; | |
| − | + | background: transparent; | |
| − | + | background-size: cover | |
} | } | ||
| + | .border { | ||
| + | background-color: #c4c4c4; | ||
| + | color: #fff; | ||
| + | padding: 18px; | ||
| + | letter-spacing: 10px; | ||
| + | opacity: 1 | ||
| + | } | ||
| + | |||
| + | .table-striped>tbody>tr:nth-of-type(odd) { | ||
| + | background-color: #f0fcff; | ||
| + | } | ||
| + | |||
| + | body { | ||
| + | margin: 0px!important; | ||
| + | padding: 0px!important | ||
| + | } | ||
table { | table { | ||
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font-size: 20px; | font-size: 20px; | ||
cell-padding: 2 10; | cell-padding: 2 10; | ||
| + | border: 1px solid #d4f4fc!important | ||
} | } | ||
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font-family: 'Open Sans', sans-serif; | font-family: 'Open Sans', sans-serif; | ||
font-size: 18px; | font-size: 18px; | ||
| + | border: 1px solid #d4f4fc!important; | ||
} | } | ||
table, th, td { | table, th, td { | ||
| − | border: 1px solid | + | border: 1px solid #d4f4fc; |
border-collapse: collapse | border-collapse: collapse | ||
} | } | ||
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padding: 0.5em; | padding: 0.5em; | ||
text-align: center | text-align: center | ||
| + | } | ||
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| + | .break { | ||
| + | word-break: break-all; | ||
| + | } | ||
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| + | h4 { | ||
| + | font-size: 35px!important; | ||
| + | font-weight: 400; | ||
| + | font-family: 'Calibri'!important; | ||
| + | padding: 2%!important; | ||
| + | text-align: center; | ||
| + | } | ||
| + | |||
| + | h2 { | ||
| + | font-size: 40px!important; | ||
| + | font-weight: 400; | ||
| + | font-family: 'Calibri'!important; | ||
| + | padding: 2%!important; | ||
| + | text-align: center; | ||
| + | } | ||
| + | |||
| + | h3 { | ||
| + | font-size: 25px!important; | ||
| + | text-align: justify; | ||
| + | text-justify: inter-word; | ||
| + | } | ||
| + | |||
| + | p { | ||
| + | text-align: justify; | ||
| + | text-justify: inter-word; | ||
| + | font-family: 'Calibri', sans-serif; | ||
| + | } | ||
| + | |||
| + | .pcontainer { | ||
| + | padding: 2%!important; | ||
| + | background: #ffffff!important; | ||
| + | color: #111; | ||
| + | border: 1px solid #ddd; | ||
| + | border-radius: .25em; | ||
| + | text-align: justify; | ||
| + | text-justify: inter-word; | ||
| + | overflow: hidden; | ||
| + | } | ||
| + | |||
| + | .plan p { | ||
| + | text-align: justify; | ||
| + | text-justify: inter-word | ||
| + | } | ||
| + | |||
| + | .plan table { | ||
| + | margin: 0.2em auto 1em; | ||
| + | font-family: 'Open Sans', sans-serif; | ||
| + | font-weight: 400 !important; | ||
| + | font-size: 16px !important; | ||
| + | line-height: 1.5 | ||
| + | } | ||
| + | |||
| + | .plan table caption { | ||
| + | text-align: center; | ||
| + | font-color: #000000!important | ||
| + | } | ||
| + | |||
| + | .plan table, th, td { | ||
| + | border: 1px solid #d4f4fc; | ||
| + | border-collapse: collapse; | ||
| + | text-align: center | ||
| + | } | ||
| + | |||
| + | .plan th, td { | ||
| + | padding: 0.5em; | ||
| + | text-align: center | ||
| + | } | ||
| + | |||
| + | body { | ||
| + | margin: 0px!important; | ||
| + | padding: 0px!important | ||
} | } | ||
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</head> | </head> | ||
<body> | <body> | ||
| − | |||
| − | <div class=" | + | <body> |
| − | + | <div style="background-color: #f5fcff!important"> | |
| − | < | + | <center> |
| − | + | <div class="col-md-12 sectionfirst" style="background-color: #f5fcff!important"> | |
| − | + | <h2 class="border" style="margin-top: 5vh; text-align: center">Part Collection</h2> | |
| − | + | </div> | |
| − | + | <div class="plan" style="background-color: #f5fcff!important"> | |
| − | + | <br><br> | |
| + | <p style="color: #f5fcff!important">f</p> | ||
| + | <br> | ||
| − | <p><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p> | + | <p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p> |
| − | <p> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p> | + | <p style="text-align: center; font-size: 20px!important"> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p> |
<br/> | <br/> | ||
| − | <p>We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p> | + | <p style="text-align: center; font-size: 20px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p> |
<br/> | <br/> | ||
| − | <p>We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p> | + | <p style="text-align: center; font-size: 20px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p> |
<br/> | <br/> | ||
Revision as of 20:23, 30 October 2017
Part Collection
f
Please visit our Part Collection page for ALL PARTS.
The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.
We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | BBa_K562001 with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. | Basic - Improved |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | BBa_K562001 with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. | Basic - Improved |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | BBa_K562001 with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. | Basic - Improved |
