Ojjonathan (Talk | contribs) |
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<p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p> | <p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p> | ||
− | <p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.</p> | + | <p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag.</p> |
+ | |||
+ | <p>This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.</p> | ||
<br> | <br> | ||
</div> | </div> |
Revision as of 20:33, 30 October 2017
Improved Part
f
Please visit our Part Collection page for ALL PARTS.
The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.
We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag.
This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.
Part Name | BioBrick | Description | Part Type |
---|---|---|---|
LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | BBa_K562001 with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. | Basic - Improved |
MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | BBa_K562001 with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. | Basic - Improved |
HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | BBa_K562001 with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. | Basic - Improved |