Difference between revisions of "Team:Lethbridge/Part Collection"

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             <h2 class="segmentHeader">Our Parts in the Registry</h2>
 
             <h2 class="segmentHeader">Our Parts in the Registry</h2>
 
   
 
   
 +
<center>
 +
<div class="partTable">
 +
<table id="partTable">
 +
    <th id="col1" style="background-color: #377b7e; color: #ffffff;">BioBrick</th>
 +
    <th id="col2" style="background-color: #377b7e; color: #ffffff;">Protein</th>
 +
    <th id="col3" style="background-color: #377b7e; color: #ffffff;">Full Construct</th>
 +
 +
<!--data rows-->
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443000" id="pageLink">BBa_K2443000</a></td>
 +
  <td class=xl94><span>AlaRS</span></td>
 +
  <td class="xl105"><span>pT7-mRBS-AlaRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443001" id="pageLink">BBa_K2443001</a></td>
 +
  <td class=xl95><span>ArgRS</span></td>
 +
  <td class="xl106"><span>pT7-mRBS-ArgRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443002" id="pageLink">BBa_K2443002</a></td>
 +
  <td class=xl94><span>AsnRS</span></td>
 +
  <td class="xl105"><span>pT7-mRBS-AsnRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443003" id="pageLink">BBa_K2443003</a></td>
 +
  <td class=xl95><span>AspRS</span></td>
 +
  <td class="xl106"><span>pT7-mRBS-AspRS-dT</span></td>
 +
</tr>
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443004" id="pageLink">BBa_K2443004</a></td>
 +
  <td class=xl94><span>CysRS</span></td>
 +
  <td class=xl105><span>pT7-mRBS-CysRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443005" id="pageLink">BBa_K2443005</a></td>
 +
  <td class=xl95><span>GlnRS</span></td>
 +
  <td class=xl106><span>pT7-mRBS-GlnRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443006" id="pageLink">BBa_K2443006</a></td>
 +
  <td class=xl94><span>GluRS</span></td>
 +
  <td class=xl105><span>pT7-mRBS-GluRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443007" id="pageLink">BBa_K2443007</a></td>
 +
  <td class=xl95><span>GlyRS alpha subunit</span></td>
 +
  <td class=xl106><span>pT7-mRBS-GlyRSAlpha-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443008" id="pageLink">BBa_K2443008</a></td>
 +
  <td class=xl94><span>GlyRS beta subunit</span></td>
 +
  <td class=xl105><span>pT7-mRBS-GlyRSBeta-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443009" id="pageLink">BBa_K2443009</a></td>
 +
  <td class=xl95><span>HisRS</span></td>
 +
  <td class=xl106><span>pT7-mRBS-HisRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443009" id="pageLink">BBa_K2443010</a></td>
 +
  <td class=xl94><span>IleRS</span></td>
 +
  <td class=xl105><span>pT7-mRBS-IleRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443011" id="pageLink">BBa_K2443011</a></td>
 +
  <td class=xl95><span>LeuRS</span></td>
 +
  <td class=xl106><span>pT7-mRBS-LeuRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443012" id="pageLink">BBa_K2443012</a></td>
 +
  <td class=xl94><span>LysRS</span></td>
 +
  <td class=xl105><span>pT7-mRBS-LysRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443013" id="pageLink">BBa_K2443013</a></td>
 +
  <td class=xl95><span>MetRS</span></td>
 +
  <td class=xl106><span>pT7-mRBS-MetRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443014" id="pageLink">BBa_K2443014</a></td>
 +
  <td class=xl94><span>PheRS alpha subunit</span></td>
 +
  <td class=xl105><span>pT7-mRBS-PheRSAlpha-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443015" id="pageLink">BBa_K2443015</a></td>
 +
  <td class=xl95><span>PheRS beta subunit</span></td>
 +
  <td class=xl106><span>pT7-mRBS-PheRSBeta-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443016" id="pageLink">BBa_K2443016</a></td>
 +
  <td class=xl94><span>ProRS</span></td>
 +
  <td class=xl105><span>pT7-mRBS-ProRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443017" id="pageLink">BBa_K2443017</a></td>
 +
  <td class=xl95><span>SerRS</span></td>
 +
  <td class=xl106><span>pT7-mRBS-SerRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443018" id="pageLink">BBa_K2443018</a></td>
 +
  <td class=xl94><span>ThrRS</span></td>
 +
  <td class=xl105><span>pT7-mRBS-ThrRS-dT</span></td>
 +
</tr>
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443019" id="pageLink">BBa_K2443019</a></td>
 +
  <td class=xl95><span>TrpRS</span></td>
 +
  <td class=xl106><span>pT7-mRBS-TrpRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443020" id="pageLink">BBa_K2443020</a></td>
 +
  <td class=xl94><span>TyrRS</span></td>
 +
  <td class=xl105><span>pT7-mRBS-TyrRS-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443021" id="pageLink">BBa_K2443021</a></td>
 +
  <td class=xl95><span>ValRS</span></td>
 +
  <td class=xl106><span>pT7-mRBS-ValRS-dT</span></td>
 +
</tr>
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443022" id="pageLink">BBa_K2443022</a></td>
 +
  <td class=xl94><span>MTF</span></td>
 +
  <td class=xl105><span>pT7-mRBS-MTF-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443023" id="pageLink">BBa_K2443023</a></td>
 +
  <td class=xl95><span>IF1</span></td>
 +
  <td class=xl106><span>pT7-mRBS-IF1-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443024" id="pageLink">BBa_K2443024</a></td>
 +
  <td class=xl94><span>IF2</span></td>
 +
  <td class=xl105><span>pT7-mRBS-IF2-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443025" id="pageLink">BBa_K2443025</a></td>
 +
  <td class=xl95><span>IF3</span></td>
 +
  <td class=xl106><span>pT7-mRBS-IF3-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443026" id="pageLink">BBa_K2443026</a></td>
 +
  <td class=xl94><span>EF-G</span></td>
 +
  <td class=xl105><span>pT7-mRBS-EFG-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443027" id="pageLink">BBa_K2443027</a></td>
 +
  <td class=xl95><span>EF-Tu</span></td>
 +
  <td class=xl106><span>pT7-mRBS-EFTu-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443028" id="pageLink">BBa_K2443028</a></td>
 +
  <td class=xl94><span>EF-Ts</span></td>
 +
  <td class=xl105><span>pT7-mRBS-EFTs-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443029" id="pageLink">BBa_K2443029</a></td>
 +
  <td class=xl95><span>RF1</span></td>
 +
  <td class=xl106><span>pT7-mRBS-RF1-dT</span></td>
 +
</tr>
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443030" id="pageLink">BBa_K2443030</a></td>
 +
  <td class=xl94><span>RF2</span></td>
 +
  <td class=xl105><span>pT7-mRBS-RF2-dT</span></td>
 +
</tr>
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443031" id="pageLink">BBa_K2443031</a></td>
 +
  <td class=xl95><span>RF3</span></td>
 +
  <td class=xl106><span>pT7-mRBS-RF3-dT</span></td>
 +
</tr>
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443032" id="pageLink">BBa_K2443032</a></td>
 +
  <td class=xl94><span>RRF</span></td>
 +
  <td class=xl105><span>pT7-mRBS-RRF-dT</span></td>
 +
</tr>
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443033" id="pageLink">BBa_K2443033</a></td>
 +
  <td class=xl95><span>MK</span></td>
 +
  <td class=xl106><span>pT7-mRBS-MK-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443034" id="pageLink">BBa_K2443034</a></td>
 +
  <td class=xl94><span>CK</span></td>
 +
  <td class=xl105><span>pT7-mRBS-CK-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443035" id="pageLink">BBa_K2443035</a></td>
 +
  <td class=xl95><span>NDK</span></td>
 +
  <td class=xl106><span>pT7-mRBS-NDK-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443036" id="pageLink">BBa_K2443036</a></td>
 +
  <td class=xl94><span>PPiase</span></td>
 +
  <td class=xl105><span>pT7-mRBS-PPiase-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/Part:BBa_K2443037" id="pageLink">BBa_K2443037</a></td>
 +
  <td class=xl95><span>T7 RNA Polymerase</span></td>
 +
  <td class=xl106><span>pT7-mRBS-T7 RNA Polymerase-dT</span></td>
 +
</tr>
 +
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/wiki/Part:BBa_K2443038" id="pageLink">BBa_K2443038</a></td>
 +
  <td class=xl94><span>tRNAPhe</span></td>
 +
  <td class=xl105><span>pT7-tRNAPhe-2xMS2 Hairpin-dT</span></td>
 +
</tr>
 +
 +
<tr>
 +
  <td class=xl100><a href="http://parts.igem.org/wiki/Part:BBa_K2443039" id="pageLink">BBa_K2443039</a></td>
 +
  <td class=xl95><span>EYFP-Spinach</span></td>
 +
  <td class=xl106><span>pT7-mRBS-EYFP-Spinach-dT</span></td>
 +
</tr>
 +
   
 +
<tr>
 +
  <td class=xl99><a href="http://parts.igem.org/Part:BBa_K2443037" id="pageLink">BBa_K2443037</a></td>
 +
  <td class=xl94><span>T7 RNA Polymerase</span></td>
 +
  <td class=xl105><span>pT7-mRBS-T7 RNA Polymerase-dT</span></td>
 +
</tr>
 +
</table>
 +
</center>
 +
</div>
 +
</div>
 +
 
<br><br>
 
<br><br>
  

Revision as of 02:08, 31 October 2017


Part Collection

The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts for future use.



Part Design Overview


Each part in our collection was designed with several features in mind:

  • Optimized for expression in E. coli
  • N- or C-terminal hexahistidine affinity tag
  • Constructs contain the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015)

Future Use for iGEM



Our Parts in the Registry

BioBrick Protein Full Construct
BBa_K2443000 AlaRS pT7-mRBS-AlaRS-dT
BBa_K2443001 ArgRS pT7-mRBS-ArgRS-dT
BBa_K2443002 AsnRS pT7-mRBS-AsnRS-dT
BBa_K2443003 AspRS pT7-mRBS-AspRS-dT
BBa_K2443004 CysRS pT7-mRBS-CysRS-dT
BBa_K2443005 GlnRS pT7-mRBS-GlnRS-dT
BBa_K2443006 GluRS pT7-mRBS-GluRS-dT
BBa_K2443007 GlyRS alpha subunit pT7-mRBS-GlyRSAlpha-dT
BBa_K2443008 GlyRS beta subunit pT7-mRBS-GlyRSBeta-dT
BBa_K2443009 HisRS pT7-mRBS-HisRS-dT
BBa_K2443010 IleRS pT7-mRBS-IleRS-dT
BBa_K2443011 LeuRS pT7-mRBS-LeuRS-dT
BBa_K2443012 LysRS pT7-mRBS-LysRS-dT
BBa_K2443013 MetRS pT7-mRBS-MetRS-dT
BBa_K2443014 PheRS alpha subunit pT7-mRBS-PheRSAlpha-dT
BBa_K2443015 PheRS beta subunit pT7-mRBS-PheRSBeta-dT
BBa_K2443016 ProRS pT7-mRBS-ProRS-dT
BBa_K2443017 SerRS pT7-mRBS-SerRS-dT
BBa_K2443018 ThrRS pT7-mRBS-ThrRS-dT
BBa_K2443019 TrpRS pT7-mRBS-TrpRS-dT
BBa_K2443020 TyrRS pT7-mRBS-TyrRS-dT
BBa_K2443021 ValRS pT7-mRBS-ValRS-dT
BBa_K2443022 MTF pT7-mRBS-MTF-dT
BBa_K2443023 IF1 pT7-mRBS-IF1-dT
BBa_K2443024 IF2 pT7-mRBS-IF2-dT
BBa_K2443025 IF3 pT7-mRBS-IF3-dT
BBa_K2443026 EF-G pT7-mRBS-EFG-dT
BBa_K2443027 EF-Tu pT7-mRBS-EFTu-dT
BBa_K2443028 EF-Ts pT7-mRBS-EFTs-dT
BBa_K2443029 RF1 pT7-mRBS-RF1-dT
BBa_K2443030 RF2 pT7-mRBS-RF2-dT
BBa_K2443031 RF3 pT7-mRBS-RF3-dT
BBa_K2443032 RRF pT7-mRBS-RRF-dT
BBa_K2443033 MK pT7-mRBS-MK-dT
BBa_K2443034 CK pT7-mRBS-CK-dT
BBa_K2443035 NDK pT7-mRBS-NDK-dT
BBa_K2443036 PPiase pT7-mRBS-PPiase-dT
BBa_K2443037 T7 RNA Polymerase pT7-mRBS-T7 RNA Polymerase-dT
BBa_K2443038 tRNAPhe pT7-tRNAPhe-2xMS2 Hairpin-dT
BBa_K2443039 EYFP-Spinach pT7-mRBS-EYFP-Spinach-dT
BBa_K2443037 T7 RNA Polymerase pT7-mRBS-T7 RNA Polymerase-dT


<groupparts>iGEM2017 Lethbridge</groupparts>