|
|
| (22 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| | {{HK_SKHLPSS_HEADER}} | | {{HK_SKHLPSS_HEADER}} |
| − |
| |
| | <html> | | <html> |
| − | | + | <img class="img-responsive my-banner" src="https://static.igem.org/mediawiki/2017/1/1b/Banner-002.jpeg"> |
| − | <div class="container" style="padding-top:60px"> | + | <div class="container" style="text-align: justify"> |
| − | <div> | + | |
| − | <img class="img-responsive" src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
| + | <h1>A Self-Assembled DNA Nano-cube for the Diagnosis of H3N2 Influenza</h1> |
| − | | + | <h2>Abstract</h2> |
| − | <!-- trash | + | |
| − | <h2> Welcome to iGEM 2017! </h2>
| + | DNA is always used as genetic material for information transfer over generations. Its programmability also allows the application in fabricating various objects for diagnostics and therapeutics as the field of DNA nanotechnology. This year, we used DNA to fabricate a three-dimensional cube that is responsive to the presence of H3N2 influenza mRNA biomarker. We used DNA and RNA oligos of the same sequence of the target for detection. From the result, we found that the DNA nano-cube specifically responses to the presence of target leading to the opening of the lid. This results in dissembling of the quadruplex formation and reduction in hemin-mediated peroxidase activity. We proved that a three-dimensional nano-device can be used for quick diagnosis within 30 minutes, and it is applicable for the detection of different biomarkers and we wish to largely produce this diagnostic device with engineered bacteria. |
| − | <p> Your team has been approved and you are ready to start the iGEM season! </p > | + | </p> |
| − | -->
| + | |
| | </div> | | </div> |
| − |
| |
| − | <h2>Abstract</h2>
| |
| − | <p>
| |
| − | A Self-Assembled DNA Nanocube for the Diagnosis of H3N2 Influenza
| |
| − | </p>
| |
| − | <p>
| |
| − | DNA is always used a genetic material for information transfer over generations. Its programmability also allows the application in fabricating various objects for diagnostics and therapeutics as the field of DNA nanotechnology. This year, we used DNA to fabricate a three-dimensional cube that is responsive to the presence of H3N2 influenza mRNA biomarker. We used DNA and RNA oligos of the same sequence of the target for detection. From the result, we found that the DNA nanocube specifically responses to the presence of target leading to the opening of the lid. This results in the disassemble of the quadruplex formation and reduction in hemin-mediated peroxidase activity. We proved that a three-dimensional nanodevice can be used for quick diagnosis within 30 minutes and it is applicable for the detection of different biomarkers and we wish to largely produce this diagnostic device with engineered bacteria.
| |
| − | </p>
| |
| − | <!--
| |
| − | The biodiversity of organism is originated from the information carried on DNA that it is only constructed by the fundamental Watson-Crick base pairing rules governing two basic combination of base pairs. The programmability of DNA allows the use of it as LEGO bricks to build different objects in both two-dimensional and three-dimensional manner.
| |
| − |
| |
| − | In addition to showing the possibility of programming DNA into different shapes, the field of DNA nanotechnology was developed to use it for medical purposes. Under the presence of target nucleic acids, conformational change of a molecular beacon that is also consisted of DNA will occur through a simple strand displacement process. Apart from nucleic acids target, presence of metal ions is also detectable by DNA molecular beacon as it affects the stability of DNA. To detect metal ion, G-quadruplex as a four-stranded nucleic acid structure is well known that the formation of it is highly depending on cation. The formation of quadruplex is able to stabilize the molecular structure of hemin and enhance its’ peroxidase activity that leads to the result of quantitative fluorescence or colorimetric signal.
| |
| − |
| |
| − | This year, the ultimate goal of our iGEM team is trying to establish a metal ion responsive molecular beacon in E. coli as a general platform for detecting environmental contamination using bacteria. In order to achieve this progressively, we will first perform the experiment in vitro using molecular beacon that consists of short oligos as a prove of concept. Then we will try to express the system in E. coli and conduct the same experiment using laboratory prepared sample and on-site collected sample for the detection of contamination.
| |
| − | -->
| |
| − |
| |
| − | <!--
| |
| − | <hr / >
| |
| − | <div class="row">
| |
| − | <div class="col-md-6">
| |
| − | <h3>Before you start: </h3>
| |
| − | <p> Please read the following pages:</p>
| |
| − | <ul>
| |
| − | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
| |
| − | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
| |
| − | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | <div class="col-md-6">
| |
| − | <div class="highlight">
| |
| − | <h3> Styling your wiki </h3>
| |
| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your
| |
| − | project.
| |
| − | </p>
| |
| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style
| |
| − | beyond what has been provided.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <hr / >
| |
| − |
| |
| − |
| |
| − | <div>
| |
| − | <h3> Wiki template information </h3>
| |
| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
| |
| − | </div>
| |
| − |
| |
| − | <hr / >
| |
| − |
| |
| − | <div class="row">
| |
| − | <div class="col-md-6">
| |
| − | <h3> Editing your wiki </h3>
| |
| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
| |
| − | <p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="col-md-6">
| |
| − | <h3>Tips</h3>
| |
| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
| |
| − | <ul>
| |
| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
| |
| − | <li>Be clear about what you are doing and how you plan to do this.</li>
| |
| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
| |
| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
| |
| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
| |
| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
| |
| − | <li>Have lots of fun! </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <hr / >
| |
| − |
| |
| − | <div class="row">
| |
| − | <div class="col-md-6">
| |
| − | <h3>Inspiration</h3>
| |
| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
| |
| − | <ul>
| |
| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
| |
| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
| |
| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
| |
| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
| |
| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
| |
| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div class="col-md-6">
| |
| − | <h3> Uploading pictures and files </h3>
| |
| − | <p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br /> When you upload, set the "Destination Filename"
| |
| − | to
| |
| − | <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
| |
| − |
| |
| − | <a href="https://2017.igem.org/Special:Upload">
| |
| − | UPLOAD FILES
| |
| − | </a>
| |
| − | </p>
| |
| − | </div>
| |
| − | </div>
| |
| − | -->
| |
| − | </div>
| |
| − |
| |
| | </html> | | </html> |
| − |
| |
| | {{HK_SKHLPSS_FOOTER}} | | {{HK_SKHLPSS_FOOTER}} |
