Difference between revisions of "Team:Hong Kong HKU/InterLab"
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<p>Green fluorescent protein, is a very commonly used measurement marker, was chosen to be measured in the study. A number of RBS test devices intended to increase the reliability and precision of gene expression were also studied. Test devices 1-6, namely BBa_J364000, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004 and BBa_J364005, along with a positive control (BBa_I20270) and a negative control (BBa_R0040) were used. All of the devices were in pSB1C3 plasmid backbones and hence, were transformed into competent E. coli (DH5-alpha) cells onto agar plates containing the antibiotic, chloramphenicol. The measurements were then performed with the help of a plate reader (Varioskan Flash 4.00.53).</p> | <p>Green fluorescent protein, is a very commonly used measurement marker, was chosen to be measured in the study. A number of RBS test devices intended to increase the reliability and precision of gene expression were also studied. Test devices 1-6, namely BBa_J364000, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004 and BBa_J364005, along with a positive control (BBa_I20270) and a negative control (BBa_R0040) were used. All of the devices were in pSB1C3 plasmid backbones and hence, were transformed into competent E. coli (DH5-alpha) cells onto agar plates containing the antibiotic, chloramphenicol. The measurements were then performed with the help of a plate reader (Varioskan Flash 4.00.53).</p> | ||
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<p> <b><a href="http://parts.igem.org/Help:Protocols/Transformation">Transformation protocol</a>:</b> </p> | <p> <b><a href="http://parts.igem.org/Help:Protocols/Transformation">Transformation protocol</a>:</b> </p> | ||
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| − | <img src="https://static.igem.org/mediawiki/2017/2/22/HKU_interlab_graph1.png" alt="<b>Figure 1: Graph showing the fluorescein fluorescence standard curve at 485 nm (excitation wavelength) and 530 nm (emission wavelength).</b>" style="width: | + | <img src="https://static.igem.org/mediawiki/2017/2/22/HKU_interlab_graph1.png" alt="<b>Figure 1: Graph showing the fluorescein fluorescence standard curve at 485 nm (excitation wavelength) and 530 nm (emission wavelength).</b>" style="width:600px;height:400px;"> |
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<p> <b>Figure 1: Graph showing the fluorescein fluorescence standard curve at 485 nm (excitation wavelength) and 530 nm (emission wavelength).</b> </p> | <p> <b>Figure 1: Graph showing the fluorescein fluorescence standard curve at 485 nm (excitation wavelength) and 530 nm (emission wavelength).</b> </p> | ||
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| − | + | <img src="https://static.igem.org/mediawiki/2017/3/3c/HKUinterlab2.png" alt="<b>Figure 2: Graph showing the fluorescein fluorescence standard curve in the log scale at 485 nm (excitation wavelength) and 530 nm (emission wavelength). </b>" style="width:600px;height:400px;"> | |
| + | <p> <b> Figure 2: Graph showing the fluorescein fluorescence standard curve in the log scale at 485 nm (excitation wavelength) and 530 nm (emission wavelength).</b> <p> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/6/6b/Interlab_Fluorescence_Graph.PNG" alt="<b>Figure 3: Graph showing fluorescence measurement of green fluorescent protein at 485nm (excitation wavelength) and 530nm (emission wavelength) over 6 hours. </b>" style="width:600px;height:400px;"> | ||
| + | <p> <b> Figure 3: Graph showing fluorescence measurement of green fluorescent protein at 485nm (excitation wavelength) and 530nm (emission wavelength) over 6 hours.</b> | ||
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| + | <img src="https://static.igem.org/mediawiki/2017/0/03/Interlab_Absorbance_Graph.PNG" alt="<b>Figure 4: Graph showing absorbance measurement of green fluorescent protein at wavelength of 600nm over 6 hours. </b>" style="width:600px;height:400px;"> | ||
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| + | <p> <b> Figure 4: Graph showing absorbance measurement of green fluorescent protein at wavelength of 600nm over 6 hours.</b><p> | ||
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Latest revision as of 16:33, 1 November 2017
InterLab
Fourth International InterLab Measurement Study
Description
This year, the HKU iGEM 2017 team participated in the Fourth International InterLaboratory Measurement Study. The iGEM Interlab study was initiated in 2014 as a way to improve the replicability and comparability of measurement data, not only in the discipline of synthetic biology but in the field of science as a whole.
Green fluorescent protein, is a very commonly used measurement marker, was chosen to be measured in the study. A number of RBS test devices intended to increase the reliability and precision of gene expression were also studied. Test devices 1-6, namely BBa_J364000, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004 and BBa_J364005, along with a positive control (BBa_I20270) and a negative control (BBa_R0040) were used. All of the devices were in pSB1C3 plasmid backbones and hence, were transformed into competent E. coli (DH5-alpha) cells onto agar plates containing the antibiotic, chloramphenicol. The measurements were then performed with the help of a plate reader (Varioskan Flash 4.00.53).
Protocols (click to access)
Plate reader measurement protocol
Results
Figure 1: Graph showing the fluorescein fluorescence standard curve at 485 nm (excitation wavelength) and 530 nm (emission wavelength).
Figure 2: Graph showing the fluorescein fluorescence standard curve in the log scale at 485 nm (excitation wavelength) and 530 nm (emission wavelength).
Figure 3: Graph showing fluorescence measurement of green fluorescent protein at 485nm (excitation wavelength) and 530nm (emission wavelength) over 6 hours.
Figure 4: Graph showing absorbance measurement of green fluorescent protein at wavelength of 600nm over 6 hours.
