Difference between revisions of "Team:Hong Kong HKU/Parts"

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<h1>Parts</h1>
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<h1>Parts</h1>
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<p>This year we produced 5 biobricks each of which produce ssDNA which can come together to form a functional tetrahedron structure in presence of its target. The inserts were ordered as gBlocks fragments from Integrated DNA technologies As shown in the diagram, the insert can be divided into 5 parts:</p>
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<ol>
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  <li>Biobrick prefix and suffix </li>
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  <li>ProD -  This is the Promoter for our biobrick. The promoter allows for high expression of our downstream product </li>
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  <li>ssDNA sequence: This sequence is complementary to the ncRNA sequence that will be produced.</li>
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  <li>HTBS: HIV reverse transcriptase binding site </li> 
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  <li>BBa_B0054 : Strong Terminator </li>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>We expect that upon transcription a ncRNA will be produced with an HTBS sequence that will allow HIV reverse transcriptase to bind the ncRNA and produce the desired DNA sequence. Upon addition of RNase H and RNase A  the DNA: RNA duplex and the degradation can occur respectively. </p>  
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<p> As shown before the Pre-tetra and the Tetra is generated using five nucleotide sequence and we have made a total of 5 biobricks, one biobrick for each. </p>  
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<center><img src="https://static.igem.org/mediawiki/parts/b/ba/Plasmidconstruct1.png" alt="plasmidconstruct" style="width:50%; height:50%" >
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<h4>Figure 1: Structure of insert</h4>
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<img src="https://static.igem.org/mediawiki/2017/7/74/Target_Vector_History.png" alt="insertadd" style="width:50%; height:50%">
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<h4>Figure 2: Production of biobrick</h4>
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<img src="https://static.igem.org/mediawiki/2017/0/0c/Webp.net-resizeimageHKU.png" alt="New gel" style="width:50%; height:50%">
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<h5>Note</h5>
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<h4>Figure 3: Polyacrylamide gel electrophoresis (PAGE) of individual oligonucleotides of our DNA nanostructure</h4>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM17 Hong_Kong_HKU</groupparts>
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<center><groupparts>iGEM17 Hong_Kong_HKU</groupparts></center>
  
 
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Latest revision as of 16:34, 1 November 2017



Parts

This year we produced 5 biobricks each of which produce ssDNA which can come together to form a functional tetrahedron structure in presence of its target. The inserts were ordered as gBlocks fragments from Integrated DNA technologies As shown in the diagram, the insert can be divided into 5 parts:

  1. Biobrick prefix and suffix
  2. ProD - This is the Promoter for our biobrick. The promoter allows for high expression of our downstream product
  3. ssDNA sequence: This sequence is complementary to the ncRNA sequence that will be produced.
  4. HTBS: HIV reverse transcriptase binding site
  5. BBa_B0054 : Strong Terminator

We expect that upon transcription a ncRNA will be produced with an HTBS sequence that will allow HIV reverse transcriptase to bind the ncRNA and produce the desired DNA sequence. Upon addition of RNase H and RNase A the DNA: RNA duplex and the degradation can occur respectively.

As shown before the Pre-tetra and the Tetra is generated using five nucleotide sequence and we have made a total of 5 biobricks, one biobrick for each.

plasmidconstruct

Figure 1: Structure of insert


insertadd

Figure 2: Production of biobrick


New gel

Figure 3: Polyacrylamide gel electrophoresis (PAGE) of individual oligonucleotides of our DNA nanostructure

<groupparts>iGEM17 Hong_Kong_HKU</groupparts>