Difference between revisions of "Team:Manchester/Basic Part"

 
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="col-md-12 sectionfirst" style="background-color: #f5fcff!important">
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<h2 class="border" style="margin-top: 5vh; text-align: center">Basic Parts</h2>
  
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<h1>Basic Parts</h1>
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<p style="color: #f5fcff!important">f</p>
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<p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p>
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<table class="table table-bordered table-striped" style="width: 1000px; max-width: 95vw">
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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</p>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<th style="background: #d4f4fc;"><b>Part Name</b></th>
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                <th style="background: #d4f4fc;"><b>BioBrick</b></th>
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                <th style="background: #d4f4fc;"><b>Description</b></th>
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<th style="background: #d4f4fc;"><b>Part Type</b></th>
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</tr>
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<td class="break">LacUV5_EutS</td>
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<td class="break"><a href="http://parts.igem.org/Part:BBa_K2213000" target="_blank"><b>BBa_K2213000</b></a></td>
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        <td>The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutS (from <i>E. coli</i>) under control of the LacUV5 inducible promoter. Contains RBS, terminators and all inducible Lac-system parts. EutS is tagged with His6.</td>
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<td>Basic</td>
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<td class="break">TetR_EutMN</td>
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<td class="break"><a href="http://parts.igem.org/Part:BBa_K2213001" target="_blank"><b>BBa_K2213001</b></a></td>
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<td>Ethanolamine Utilisation (Eut) bacterial micro-compartment (BMC) proteins EutM and EutN from <i>E. coli</i>, placed under the inducible Tetracycline promoter. Contains RBS, terminators and all tetp components. EutM is tagged with GFP and His6. EutN is tagged with FLAG.</td>
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<td>Basic</td>
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<td class="break">araBAD_EutLK</td>
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<td class="break"><a href="http://parts.igem.org/Part:BBa_K2213002 " target="_blank"><b>BBa_K2213002</b></a></td>
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<td>The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutLK (from <i>E. coli</i>) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG. </td>
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<td>Basic</td>
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<td class="break">cgPPK2_His6</td>
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<td class="break"><a href="http://parts.igem.org/Part:BBa_K2213003" target="_blank"><b>BBa_K2213003</b></a></td>
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<td><i>Corynebacterium</i> PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator.</td>
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<td>Basic</td>
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</tr>
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<td class="break">PduD(1-20)_cgPPK2_mCherry_His6</td>
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<td class="break"><a href="http://parts.igem.org/Part:BBa_K2213004" target="_blank"><b>BBa_K2213004</b></a></td>
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<td><i>Corynebacterium</i> PPK2 with a C terminal mCherry-polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator.</td>
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<td>Basic</td>
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</tr>
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<td class="break">PduD(1-20)_mCherry_cgPPK2_His6</td>
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<td class="break"><a href="http://parts.igem.org/Part:BBa_K2213005" target="_balnk"><b>BBa_K2213005</b></a></td>
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<td><i>Corynebacterium</i> PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag and mCherry at the N terminal. Contains RBS and terminator.</td>
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<td>Basic</td>
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<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>,with the illegal XbaI site removed, and with a low promoter (<a href="http://parts.igem.org/Part:BBa_J23105" target="_blank"><b>BBa_J23105</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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<td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a medium promoter (<a href="http://parts.igem.org/Part:BBa_J23108" target="_blank"><b>BBa_J23108</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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</tr>
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<td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a high promoter (<a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"><b>BBa_J23104</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry_cgPPK2</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213009"target="_blank"><b>BBa_K2213009</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a> with cgPPK2 added to the C terminus.</td>
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<td style="border:1px black solid;">Basic</td>
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<h3>Best Basic Part Special Prize</h3>
 
  
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Composite_Part " ><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Manchester--NEXTARROWPARTS17.jpg"></a>
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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// Create NGL Stage object
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<h4>Note</h4>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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{{:Team:Manchester/Templates/Footer}}

Latest revision as of 21:02, 1 November 2017

HOME
TEAM
PROJECT
Overview
Description
Results
Demonstration
Medal Criteria
Achievement
NOTEBOOK
Protocols
Diary
Lab Book
InterLab
Measurement
PARTS
Parts
Basic Parts
Composite Parts
Improved Parts
Part Collection
HUMAN PRACTICES
Silver HP
Water Industry
Integrated and Gold
Intellectual Property
Public Engagement
Entrepreneurship
MODELLING
Overview
D. O. Experiment
Continuous Culture
PHO Operon
JUDGING FORM

Basic Parts

f


Please visit our Part Collection page for ALL PARTS.


Part Name BioBrick Description Part Type
LacUV5_EutS BBa_K2213000 The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutS (from E. coli) under control of the LacUV5 inducible promoter. Contains RBS, terminators and all inducible Lac-system parts. EutS is tagged with His6. Basic
TetR_EutMN BBa_K2213001 Ethanolamine Utilisation (Eut) bacterial micro-compartment (BMC) proteins EutM and EutN from E. coli, placed under the inducible Tetracycline promoter. Contains RBS, terminators and all tetp components. EutM is tagged with GFP and His6. EutN is tagged with FLAG. Basic
araBAD_EutLK BBa_K2213002 The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG. Basic
cgPPK2_His6 BBa_K2213003 Corynebacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator. Basic
PduD(1-20)_cgPPK2_mCherry_His6 BBa_K2213004 Corynebacterium PPK2 with a C terminal mCherry-polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator. Basic
PduD(1-20)_mCherry_cgPPK2_His6 BBa_K2213005 Corynebacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag and mCherry at the N terminal. Contains RBS and terminator. Basic
LowPromoter_PduD(1-20)_mCherry BBa_K2213006 BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. Basic - Improved
MediumPromoter_PduD(1-20)_mCherry BBa_K2213007 BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. Basic - Improved
HighPromoter_PduD(1-20)_mCherry BBa_K2213008 BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. Basic - Improved
LowPromoter_PduD(1-20)_mCherry_cgPPK2 BBa_K2213009 BBa_K2213006 with cgPPK2 added to the C terminus. Basic