Difference between revisions of "Team:Manchester/Basic Part"
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| + | float: right!important; | ||
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| + | .table-striped>tbody>tr:nth-of-type(odd) { | ||
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table, th, td { | table, th, td { | ||
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.plan table, th, td { | .plan table, th, td { | ||
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border-collapse: collapse; | border-collapse: collapse; | ||
text-align: center | text-align: center | ||
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<body> | <body> | ||
| − | < | + | <div class="clear"></div> |
| − | <div class="col-md-12 sectionfirst" style="background-color: # | + | <div class="plan" style="background-color: #f5fcff!important"> |
| + | <center> | ||
| + | <div class="col-md-12 sectionfirst" style="background-color: #f5fcff!important"> | ||
<h2 class="border" style="margin-top: 5vh; text-align: center">Basic Parts</h2> | <h2 class="border" style="margin-top: 5vh; text-align: center">Basic Parts</h2> | ||
</div> | </div> | ||
| − | < | + | |
| + | <div class="clear" style="background-color: #f5fcff!important; padding-top: 10vh"> | ||
| + | <p style="color: #f5fcff!important">f</p> | ||
| + | <br> | ||
<p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p> | <p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p> | ||
<br> | <br> | ||
| Line 151: | Line 166: | ||
<tr> | <tr> | ||
| − | <th style="background: # | + | <th style="background: #d4f4fc;"><b>Part Name</b></th> |
| − | <th style="background: # | + | <th style="background: #d4f4fc;"><b>BioBrick</b></th> |
| − | <th style="background: # | + | <th style="background: #d4f4fc;"><b>Description</b></th> |
| − | <th style="background: # | + | <th style="background: #d4f4fc;"><b>Part Type</b></th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td class="break"><a href="http://parts.igem.org/Part:BBa_K2213004" target="_blank"><b>BBa_K2213004</b></a></td> | <td class="break"><a href="http://parts.igem.org/Part:BBa_K2213004" target="_blank"><b>BBa_K2213004</b></a></td> | ||
<td><i>Corynebacterium</i> PPK2 with a C terminal mCherry-polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator.</td> | <td><i>Corynebacterium</i> PPK2 with a C terminal mCherry-polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator.</td> | ||
| − | <td | + | <td>Basic</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td | + | <td class="break">PduD(1-20)_mCherry_cgPPK2_His6</td> |
| − | <td | + | <td class="break"><a href="http://parts.igem.org/Part:BBa_K2213005" target="_balnk"><b>BBa_K2213005</b></a></td> |
| − | <td | + | <td><i>Corynebacterium</i> PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag and mCherry at the N terminal. Contains RBS and terminator.</td> |
| − | <td | + | <td>Basic</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td> | ||
| − | <td style="border:1px black solid;"> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>,with the illegal XbaI site removed, and with a low promoter (<a href="http://parts.igem.org/Part:BBa_J23105" target="_blank"><b>BBa_J23105</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
</tr> | </tr> | ||
| Line 201: | Line 216: | ||
<td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td> | ||
| − | <td style="border:1px black solid;"> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a medium promoter (<a href="http://parts.igem.org/Part:BBa_J23108" target="_blank"><b>BBa_J23108</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
</tr> | </tr> | ||
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<td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td> | ||
| − | <td style="border:1px black solid;"> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a high promoter (<a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"><b>BBa_J23104</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
<tr> | <tr> | ||
<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry_cgPPK2</td> | <td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry_cgPPK2</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213009"target="_blank"><b>BBa_K2213009</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213009"target="_blank"><b>BBa_K2213009</b></a></td> | ||
| − | <td style="border:1px black solid;">BBa_K2213006 with cgPPK2 added to the C terminus.</td> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a> with cgPPK2 added to the C terminus.</td> |
<td style="border:1px black solid;">Basic</td> | <td style="border:1px black solid;">Basic</td> | ||
</tr> | </tr> | ||
| − | |||
</table> | </table> | ||
</center> | </center> | ||
| + | <br> | ||
| − | < | + | <div class="plan" style="padding: 0px 8vw; background-color: #f5fcff!important"> |
| − | < | + | <a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Composite_Part " ><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Manchester--NEXTARROWPARTS17.jpg"></a> |
| − | + | <br> | |
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
</div> | </div> | ||
| + | </div> | ||
| + | </div> | ||
</body> | </body> | ||
| + | |||
| + | <script> | ||
| + | $(document).ready(function() { | ||
| + | |||
| + | // Create NGL Stage object | ||
| + | var stage = new NGL.Stage( "viewport" ); | ||
| + | |||
| + | // Handle window resizing | ||
| + | window.addEventListener( "resize", function( event ){ | ||
| + | stage.handleResize(); | ||
| + | }, false ); | ||
| + | |||
| + | |||
| + | // Load PDB entry 1CRN | ||
| + | stage.loadFile( "https://2017.igem.org/Team:Manchester/ppk.pdb", { defaultRepresentation: true } ).then(function (o) { | ||
| + | o.addRepresentation("cartoon"); | ||
| + | stage.autoView(); // focus on all representations in all components | ||
| + | }); | ||
| + | |||
| + | }); | ||
| + | </script> | ||
| + | |||
</html> | </html> | ||
{{:Team:Manchester/Templates/Footer}} | {{:Team:Manchester/Templates/Footer}} | ||
Latest revision as of 21:02, 1 November 2017
Basic Parts
f
Please visit our Part Collection page for ALL PARTS.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LacUV5_EutS | BBa_K2213000 | The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutS (from E. coli) under control of the LacUV5 inducible promoter. Contains RBS, terminators and all inducible Lac-system parts. EutS is tagged with His6. | Basic |
| TetR_EutMN | BBa_K2213001 | Ethanolamine Utilisation (Eut) bacterial micro-compartment (BMC) proteins EutM and EutN from E. coli, placed under the inducible Tetracycline promoter. Contains RBS, terminators and all tetp components. EutM is tagged with GFP and His6. EutN is tagged with FLAG. | Basic |
| araBAD_EutLK | BBa_K2213002 | The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG. | Basic |
| cgPPK2_His6 | BBa_K2213003 | Corynebacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator. | Basic |
| PduD(1-20)_cgPPK2_mCherry_His6 | BBa_K2213004 | Corynebacterium PPK2 with a C terminal mCherry-polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator. | Basic |
| PduD(1-20)_mCherry_cgPPK2_His6 | BBa_K2213005 | Corynebacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag and mCherry at the N terminal. Contains RBS and terminator. | Basic |
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. | Basic - Improved |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. | Basic - Improved |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. | Basic - Improved |
| LowPromoter_PduD(1-20)_mCherry_cgPPK2 | BBa_K2213009 | BBa_K2213006 with cgPPK2 added to the C terminus. | Basic |
